Jackass Number Two is a compilation of various stunts, pranks and skits, and essentially has no plot. The film opens with an introduction of the nine cast members while they're being chased by bulls in a neighborhood. One by one, the cast members are taken down by the stampede, until only Johnny Knoxville and Bam Margera are left, and are chased through a house. Bam jumps through a window, and Johnny stops in his tracks to deliver his signature line, "Hi, I'm Johnny Knoxville, welcome to Jackass!", and is pushed through a window by several bulls, and the title comes to screen, "Jackass Number Two".
The movie finishes with a Busby Berkeley-style movie musical production number set to the La Cage aux Folles song "The Best Of Times", where the cast sing and dance while getting battered by violent stunts. One stunt involves Wee Man and Preston Lacy sliding onto a bunch of metal trash cans. Others involve Chris Pontius dancing in a flaming building and jumping safely onto a mat, at which point he is knocked off his feet by a fire hose, and Bam and Dunn dancing in cowboy outfits in front of a horse before Knoxville causes the horse to flee by slapping its rear, pulling Bam and Dunn away by a rope attached to their feet. As in the first film, Rip Taylor is seen at the end of the sequence.
Leading up to the production number at the end, the following sequences and scenes are performed.
1. Puppet Show: Knoxville puts on a puppet show using a snake and Pontius' penis.
2. The Valentine: A construction paper heart with a note on it is hung in a hotel room hallway, with smaller and smaller letters as the note continues.
3. Firehose Rodeo: Dave England rides an out of control fire hose.
4. Chubby Chaser India: Preston chases Wee Man through the streets of an Indian town and deals with a bunch of little problems.
5. Bicentennial BMXing: Knoxville and Dunn attempt to perform stunts on 18th century style bicycles.
6. The Strongman: Bam sits on top of a strength tester machine while Pontius sends a golden dildo rocketing towards his rear.
7. Gloria: Spike Jonze plays a 90-year old prostitute named Gloria who loses her top out on the street.
8. The Mini-Loop: Danger Ehren McGhehey and Thor Drake try to ride a miniature motorbike through a wooden loop.
9. The Brand: Dunn brands Bam with a penis-shaped branding iron.
10. Lake Jump: The guys use rocket-propelled vehicles to try and jump a lake.
11. The Fish Hook: Pontius fishes for sharks in the Gulf of Mexico using Steve-O as his bait.
12. Bam Velcro Truck: Bam tries to stick himself to a truck while wearing a velcro suit.
13. The Electric Stool: Professional card thrower Jim Karol and Steve-O trick Wee Man into thinking he's getting cards thrown into his rectum while he sits on an electrically-rigged chair.
14. Yak Charge: Knoxville is gored by a yak.
15. The Bungee Jump: Wee Man bungee jumps off a bridge using Preston as his support weight.
16. Bad Grandpa: Knoxville dresses up as an old man named Irving Zisman, who gives a kid cigarettes and booze.
17. Indoor Ski Slope: April and Phil Margera's hallway stairs are turned into a ski slope by the guys.
18. Beehive Limo: Knoxville and Bam trick Wee Man, Dave, Steve-O, and Dunn by hiring a limo to come to a fake photo shoot and pouring bees through the sunroof, then securing the child locks so they can't get out.
19. Rake Jump: Steve-O jumps on a rake.
20. Dave Eats Shit: The Three 6 Mafia pays Dave $200 to eat horse feces. (The unrated cut of the film featured Dave being paid a larger sum to try and eat a larger piece of fecal matter.)
21. Naked Wee Man: Wee Man walks through a production meeting in the buff.
22. Riot Control Test: The guys become test subjects for riot control ammunition.
23. Gloria and the Artist: Gloria tries to buy a painting from a streetcorner art salesman, but can't keep her saggy breasts from popping out.
24. Medicine Ball Dodgeball: The guys play a game of dodgeball in the dark using medicine balls.
25. Mushroom Launch: Dunn is about to ride his shopping cart out of a loading dock, but Bam has other ideas.
26. The Magic Trick: John Waters makes Wee Man disappear.
27. The Gauntlet: Mat Hoffman, Tony Hawk, and the guys try to negotiate a skate park ramp while dodging swinging sandbags.
28. Tiny Toilet: Dave takes a dump in a dollhouse-sized bathroom.
29. Toro Totter: Knoxville, Bam, Pontius, and Dunn ride a teeter-totter while a bull charges.
30. Keep God out of California: Pontius dresses as the devil and shoots out from below the ground to protest God.
31. Butt Chug: Steve-O shotguns a beer in his anus.
32. Bam Roof Slam: Bam hurts himself skateboarding off a roof.
33. Rocket Cart: Dunn tries to jump the lake in his cart, which is now rocket propelled.
34. Anaconda Ball Pit: Knoxville, Dunn, and Wee Man try to fish an anaconda out of a children's ball pit.
35. Bam Drop In: Bam drops in and hits the camera.
36. The Ice Horse: Knoxville and CKY's Brandon DiCammillo dare Dunn to sit on a horse-shaped ice sculpture with his scrotum hanging out.
37. The Swamp Chute: Wee Man is launched off a dock using high powered fans.
38. How to Milk a Horse: Knoxville, Pontius, and Steve-O obtain a sperm sample from a studded horse.
39. Karate Chop: Dunn holds wooden boards while Pontius and Bam try to break them.
40. The Big Tire Race: Danger Ehren and Dave race each other down a hill while riding in monster truck tires.
41. The Leech Healer: The boys find a leech healer in India and Dave and Steve-O need healing. (Note: Dave's scene is only available in the unrated DVD version.)
42. Gloria Goes Shopping: Gloria again has trouble keeping her breasts in her top, this time while shopping.
43. The Wind Tunnel: Bam attempts to withstand high powered fans while he hangs on to a horse trailer. A king cobra throws a wrench in those plans.
44. Roller Leapfrog: Costumed mascots try to play leapfrog in a roller rink.
45. The Fart Mask: Steve-O puts on a helmet that Preston farts and defecates into.
46. Big Green Ball: Dave jumps off a roof while riding a bouncing ball.
47. The Poof: Irving farts baby powder into Jeff Tremaine's face.
48. Old Man Balls: Irving's testicles won't stay in his shorts.
49. The Switcheroo: Phil and Preston trade places in the middle of the night to try and fool April.
50. Ding Dong: Dave visits Knoxville's house, and gets hit by a giant inflatable object when he rings the doorbell.
51. Big Red Rocket: Knoxville rides a red rocket over the lake.
52. Terror Taxi: The guys play a prank on Danger Ehren, who they dress up as a suicide bomber. A gun-toting Jay Chandrasekhar helps and many of the guys make special contributions to the costume.
53. The Bear Trap: Knoxville puts his arm in a bear trap.
