Originally Jensen Ackles auditioned for the role of Sam Winchester. When Jared Padalecki came in and read for Sam a slight recasting was done. Producers called Jensen and asked if he'd rather use his charm for Dean, saying he was the Han Solo character of the duo and what kid didn't grow up wanting to be Han Solo? Jensen accepted. It became known that he was previously interested in that role, because Dean was funny.
While it maybe coincidental the choice of the name Winchester for the family is interesting. There is a rather famous mansion in California built by Sarah Winchester, wife of the gun magnate, which was under constant construction for 38 years for paranormal reasons. According to legend the reason for the constant building was a medium telling Mrs. Winchester that if she ever stopped building the spirits of those killed by Winchester rifles would come after her. The strange design of the mansion is also attributed to this. It was built with dead ends and stairs to nowhere allegedly to confuse spirits.
Early on in the series, Dean's cell number is given, and for a while the studio had it set up so that anyone dialing the number would hear Jensen Ackles reading the message: "This is Dean Winchester. If this is an emergency, leave a message. If you are calling about 11-2-83, page me with your coordinates." The number is 1-866-907-3235. This number now leads to a fax machine, but it is not known where that fax machine ends up.
None of the cast or crew members ever expected for show to last as long as it has. Misha Collins (Castiel) has stated that at the 100th episode party, someone said "Maybe we'll see you at the 200th!" and everyone laughed at the idea, thinking the show would be long gone by that time. The party for the 200th episode of the series took place in October 2014.
Riverview Hospital, a large mental health facility used as the main set in the season 1 episode Asylum, has also substituted for a prison, a regular hospital, and an apartment building on different episodes of the show. This is evidenced by seeing the same distinctive staircase in different episodes.
An important aspect of the show's music is classic rock, most of which comes from creator Eric Kripke's own record collection, although one of his favorite bands - Led Zeppelin - has always been too expensive to license.
Every single season finale begins with a recap montage of the key moments of the season, to the tune of "Carry On Wayward Son." The exception was season 1 when the song is heard in penultimate episode of the season.
Many episodes are either named after movies ("Children Shouldn't Play with Dead Things", "The Usual Suspects", "I Know What You Did Last Summer") or classic rock songs ("In My Time of Dying", "Born Under a Bad Sign", "What is and What Should Never Be", "Sympathy For The Devil", "When The Levee Breaks").
Jared Padalecki (Sam Winchester) dislocated his shoulder while wrestling with Osric Chau (Kevin Tran). The writers were then forced to make up a story as to why Sam was wearing a sling in the beginning of Season 10.
To keep their cover as fighters of the Supernatural, Sam and Dean Winchester often pose as agents from varying agencies (FBI, Homeland Security, CDC, etc). The pseudonyms they use as officials are often direct allusions to rock stars, such as agents "Angus and Young," an homage to Angus Young from AC/DC or agents "Tyler and Perry," an allusion to Steven Tyler and Joe Perry of Aerosmith. Several times in particular Dean refers to himself as "Van Halen," an allusion to any member of the famous '80s rock band. In later seasons, the Winchester brothers refer to themselves as agents "Plant and Page," an allusion to the members of "Led Zeppelin," (Eric Kripke's' favorite band) or agents "Shaw and DeYoung," an allusion to the members of "Styx," some of whose music is featured in the series.
Many of the episodes are based on existing legends, myths, and ghost stories. For example the episode "Crossroads" is based in part on the legend of Robert Johnson - who in turn borrowed it from another Blues singer.
The character Lilith, a white-eyed demon introduced in season 4, is the name of the child of Aleister Crowley. It is also the name of Adam's first wife in certain traditions, who left paradise and consorted with demons, and is sometimes referred to as the mother of demons.
Both Jared Padalecki and Jensen Ackles auditioned for the role of Superman/Clark Kent in some form. Padalecki was a finalist for director McG's reboot while Ackles was the second choice to play a young Clark Kent in the show Smallville (2001). Jensen was later cast as Jason Teague in Season 4.
Misha Collins (Castiel) was originally only supposed to make a couple of guest appearances in season four, but due to enormous fan response, he has been a regular on the show ever since. Collins has joked that he maybe wouldn't have used such a low gravelly voice for his role had he known he'd be on the show so frequently because it hurts his throat.
Dean's car, up until episode 2.20, had Kansas (the brother's origin state) plates with the number KAZ 2Y5. It currently now has Ohio (Supernatural's creator Eric Kripke's home state) plates with the number CNK 80Q3. The plates are changed again in later seasons to Oregon plates SMD 5B2, as seen in episode 13 of season 7.
Eric Kripke is a big fan of Classic Rock, and thus wanted it to be played on the show. He wrote in the pages of the pilot script "CUE MUSIC And you can take your anemic alternative pop and shove it up your ass. Dean plays bass thumping, pile driving Zeppelin, and he plays it loud."
The Supernatural fan base is repeatedly referenced throughout the show and even makes a few special appearances, including the 200th episode. This was made possible through the character Chuck Shurley and his prophetic book series about the Winchesters.
The character of Bobby Singer was originally supposed to be named Bobby Manners, after Robert Singer and Kim Manners, who had both greatly contributed to the show. However, after discovering that there was an actual Bobby Manners, the character's last name was changed to Singer.
Whenever the brothers are introduced (by their real names) by themselves or others they are always referred to as Sam and Dean and Never as Dean and Sam. This is a nod to Jared Padalecki's name being displayed first in the opening credits.
This is the second time Jensen Ackles plays a sibling to a character named Sami/Sammy as he previously appeared on the daytime soap "Days of Our Lives" as Eric, twin brother to Alison Sweeney's Samantha Brady. Sweeney's character usually went by the name Sami. Ackles' character tends to call his brother Sam, "Sammy" on Supernatural.
In season 2, episode 12, Night Shifter, the magazine that Ron shows Sam and Dean (disguised as FBI agents) has a picture of a humanoid metal robot, or "Mandroid" as Ron insists on calling it, with the caption "Cyberman". Both the name "cyberman" and the likeness of the robot are references to Docor Who, with the cybermen being prominent antagonists in the show.
Sebastian Roché (Balthazar), Misha Collins (Castiel), and Mark Sheppard (Crowley) all appeared in the TV series "Charmed." Roché and Sheppard both played demonic characters, while Collins played a character in need of help.
The trivia items below may give away important plot points.
Producers considered several methods of motivating Sam to take to the road with Dean. Turning Jessica evil was an option briefly, but in taking a page from Star Wars it was decided that her death would be the ultimate motivation for leaving home.
Several references throughout his appearances on the series point toward the character Rufus Turner (played by Steven Williams) being Jewish. He makes jokes about Hanukkah and keeping kosher, and most significantly, when he dies, the Winchester brothers and Bobby give him a funeral in a Jewish cemetery and discuss his level of Jewish ritual observance (or lack thereof). Although the funerals for previous hunters had included a ceremonial funeral pyre, the show makes a point of showing that Rufus gets buried without cremation (since cremation is against Jewish tradition).
Genevieve Cortese' was cast as the character Ruby for the fourth season (replacing Katie Cassidy), and her character eventually becomes romantically involved with Sam (Jared Padalecki). In their real lives, Cortese and Padalecki also became romantically involved, and after they married in 2010, she changed her last name to "Padalecki" both personally and professionally.
The character Sam was sick for a large part of Season 8 due to the Demon Trials, and actor Jared Padalecki's performance was so convincing that a portion of fans became concerned about him, worrying that he was actually sick.
In The Girl Next Door episode there's the scene where a leviathan is checking over a credit card statement and asks about "Mistress Magda", before hanging up on the customer, because of getting a trace on Dean and Sam. Mistress Magda is the name Chuck says when he answers the phone in Swan Song when Dean calls for the location of the fight between Michael and the Devil, the last time we see Chuck and he mysteriously disappears in a puff of white smoke.
In the show, Chuck (a prophet of the Lord) uses a pen name to write the Supernatural books: Carver Edlund. The producer's name is Ben Edlund and the co-producer's name is Jeremy Carver. Putting the last names together results with Chuck's pen name.