- DIRECTOR CAMEO ('James Gunn'): There's a quick conversation at the school with Starla and another teacher, played by James Gunn.
- The books that twins Jenna and Emily read are two popular Goosebumps books: "You Can't Scare Me" and "The Girl Who Cried Monster".
- Jenna and Emily Strutemyer were named after Jenna Fischer, director James Gunn's wife, and her sister.
- Actors Nathan Fillion and Gregg Henry were both on the TV show _Firefly_ . Fillion starred as Malcolm Reynolds, and Henry guest-starred as Sheriff Bourne in the episode "The Train Job".
- There is a mention of neighbors named "the Castevets". The Castevets were Rosemary's neighbors in Rosemary's Baby (1968).
- As an in-reference to past genre movies, the Mayor and his store are named R.J. MacReady, the same name as Kurt Russell's character in John Carpenter's The Thing (1982), and the high school is named after Earl Bassett (Fred Ward's character from Tremors (1990).
- The ammunition store is named for Max Renn from Videodrome (1983).
- The bar hosting the "Deer Cheer" party is called "Henenlotter's", a homage to Basket Case (1982) director Frank Henenlotter.
- When Starla is on the phone talking to the doctor about Grant, the voice of the doctor is Rob Zombie.
- Grant's doctor (Dr. Carl) is an homage to Dr. Karl, a small character from David Cronenberg's Rabid (1977).
- Kylie bashing open the twins bedroom door with a horse head is a tribute to the slasher/comedy flick Student Bodies which included murder and door bashing by shop class made horse head book ends.
- Haig Sutherland was the first person to be cast. Elizabeth Banks was the second. Nathan Fillion was the last actor to be cast, about a week before shooting began.
- More than 100 actors in L.A. and Vancouver auditioned for the role of Jack MacReady. Gregg Henry was the last actor to be seen for the part and nailed his audition.
- The shoot lasted 47 days.
- When Grant reaches Brenda James' house, she's watching The Toxic Avenger (1985) on TV.
>>> WARNING: Here Be Spoilers <<<
Trivia items below here contain information that may give away important plot points. You may not want to read any further if you've not already seen this title.
- SPOILERS: The post-credits coda shows a cat getting possessed by the last surviving creature. In an early draft, this was meant to happen to Bill, after the Grant-creature is destroyed. Starla sees the resulting wound later, prompting her to shoot Bill to death.
- SPOILER: Actress Jenna Fischer disclosed on a TV talk show that she got the role of Shelby as a "birthday present" from her spouse, director James Gunn, after another actress dropped out of the movie. Being a big fan of zombie films, she always wanted to play a zombie; upon hearing the news, she screamed with joy.
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