The truck carrying the fish at the beginning of the film has an out-of-date Florida license plate, the kind that was issued between 1978 and 1985 and has been long obsolete. Also, there is no county name/'Sunshine State' on the bottom like all plates have.
One of the stickers on the cab's windshield expires 04/02, Legislation creating the Department of Homeland Security wasn't enacted until 11/02 and the actual date for the creation of the Department of Homeland Security was March 1, 2003. In other words, the Department of Homeland Securty did not exist until March 1, 2003. As closely as cabs are regulated at the airport this seems unlikely for the cab to get by for so long with an expired registration or inspection.
When Jack sees Lisa in the airport reading the magazine while hiding from the police there are two men sitting at a table having drinks, but when Jack starts to run after her the men and the table are both gone.
When Lisa runs out of the plane after the aircraft door opened, you can see a ramp agent in the scene. But just two seconds after, when the flight attendant runs to the same place, the ramp agent is gone.
When Jack is holding Lisa's head in the airplane restroom, he is holding her cheeks. In the side view, his thumb is in the middle (center of her jaw). But when it cuts to the close up, Jack's thumb should be visible but it isn't as it is much further back. It then goes back to the side view and his hand is in the center again.
When Lisa and Jackson run upstairs in the chase scene through the house, Lisa runs through a room where bright sunlight is coming from the windows before closing the door behind her, meaning these windows were open. After Jackson picks the lock and swings the door open, you can see that the curtains are shut and the room is dark.
In the scene where Lisa rams into the hitman with a Jeep Cherokee, the SUV's antenna is attached as he tumbles onto the hood, but is missing in an oblique camera shot just before the car impacts the doorway, then reappears in the roof camera shot at impact, then is gone again in the final oblique camera shot after the car has rebounded from the doorway.
As they approach the gate to board the aircraft, we can see a tri-jet through the window. This would be a Boeing 727, McDonnell Douglas DC-10 or Lockheed L-1011. Once aboard the plane, the interior is that of a Boeing 767. Shots of the plane taxiing are of an Airbus A320. Take-off and landing shots show a Boeing 737. Exterior shots of the plane in flight are of a Boeing 737.
When Jackson is chasing Lisa upstairs in her father's house, she locks herself in the bathroom. While she is at the door, the shower curtain to the right is halfway open. Before she opens the curtain to see if Jackson is hiding in the shower, the curtain is closed.
When Lisa is in the stolen car from the airport, the scene shows her with her seat beat strap across her shoulder when she she uses her cell phone, but when she swerves to miss a car she reaches for her seat belt over her left shoulder and buckles it.
When Jackson's associate grabs Lisa's father's wallet in the beginning we can see a picture of her in Lacross, there is someone else in the photo, but later on in the movie when we see the picture again there is just Lisa.
When Lisa offers the book to the old woman in the line in front of her, she's holding the book right side up. However, when the woman takes the book, it's upside down, but a few shots later it's right side up again.
Throughout the film, you can see that Jackson Rippner's eyebrows are visibly thicker or thinner in some scenes. Due to playing a transgendered male in Breakfast on Pluto prior to Red Eye, Cillian Murphy (Jackson) had had his eyebrows tweezed for that role.
Although the airplane interior is a "standard" Boeing 767 layout (2-3-2), row 18 is shown too far back in the plane. The row used in film should be numbered closer to 30 or so as these rows are closest to the back of the plane and the rear galley.
The cab that drops Lisa off has three stickers on the windshield on the passenger side and none on the driver's side. These surely are supposed to be registration and inspection stickers which are required to be on the driver's side according to Texas law.
The personnel on the Coast Guard patrol vessel checking out the yacht in the harbor wore Shore Patrol vests. The Navy, Marine Corps and Coast Guard use Shore Patrol personnel, who are untrained in law enforcement, to maintain order on land among its own enlisted members. The Coast Guard uses trained personnel for law enforcement duty on the seas.
In the scene where the Lisa rams the hitman with a Jeep and launches him through the door, immediately after the camera cuts to the interior of the house, cuts are visible in the wood of the doorway to allow it to break away.
You can see when Jackson is throwing Lisa against the walls in the bathroom that he really has his hand behind her head. It was later revealed that Cillian Murphy did this on purpose. In an earlier take, he accidentally knocked Rachel McAdams out and wanted to prevent it from happening again.
At the beginning of the film when Jackson Rippner's partner is going through Joe Reisert's wallet, he pulls out Joe Reisert's drivers license. The street address given is on Maryland Avenue, yet Jackson Rippner recites a different address to Lisa Reisert.
In addition to the different planes shown, the final exterior shot as the plane is taking off is actually a 737 landing. The flaps are clearly in the landing configuration and the reverse thrusters are on.
At the end of the movie, Lisa shoots Jackson and hits him on the left side of his midsection. Several seconds later, Lisa's father shoots him again. The first bloodstain is gone and has been replaced by one centered on his upper chest area. When Jackson is seen lying on the ground, there is just one bloodstain showing near his right collarbone/throat area.