While The Batman faces off against Temblor, a villain possessing seismic powers, we meet Commissioner Gordon's teenage daughter, Barbara. Already worried his daughter lacks focus, Gordon fears Barbara's new friend Pam Isley is a bad influence.
Professor Hugo Strange, head of Arkham Asylum, replaces Arnold Wesker's villainous ventriloquist puppet, Scarface, with a "benign" puppet named Mr. Snoots. With the positive Mr. Snoots on his arm, Wesker is able to begin rebuilding his life.
Gearhead, a part-cyborg adrenaline junkie, rides into Gotham in a souped-up racer. With the ability to take control of cars with his nano-technology, Gearhead is out to commit a series of crimes for thrills.
After getting his hands on Bane's steroid apparatus, Joker uses it to bulk up to monstrous proportions. As Joker toys with the city with his superhuman strength, The Batman and Batgirl must find a way to defeat this unstoppable force.
When her father, along with other high-ranking Gotham officials, begins to embrace environmental causes after the delivery of mysterious plants, Barbara discovers that they've been replaced with plant-based copies created by Poison Ivy and teams up with Batman to stop her and their own evil clones.
After losing the mayoral election, billionaire technologies' mogul Maximilian Zeus takes to the skies in an airship in order to rule over Gotham like a god. Employing the Batwing and getting an assist from Batgirl, who's made her way onto the ship, Batman tries to bring the ego-maniacal Zeus back to earth.
Joker takes on an apprentice who is a school mate (class clown) of Batgirl. In the end, when given the opportunity to kill batman and bat-girl the apprentice boy, can't go through with it. Batman and girl save the boy for boiling vats of acid and the boy knocks out joker with his own weapon.
When Alfred joins Ewan Fripp, an "old school chum" and world renowned treasure hunter, on his latest adventure, the pair find themselves in a race with Penguin and Mr. Freeze to locate a fortune which has been long hidden somewhere in Gotham.
The Digitally Advanced Villain Emulator, or D.A.V.E., is an artificially intelligent computer program made up of the combined psychological profiles of the most diabolical criminals committed to Arkham Asylum.