According to David Slade, veteran director Sam Raimi was slated to direct the film when the script was in its earliest stages; then Raimi opted to produce instead.
Steve Niles originally conceived the story as a film. After years of rejection by studios, it was reworked into a comic book. Eventually, a studio that rejected the original screenplay went for the comic version.
According to the DVD commentary, Josh Hartnett was sick with mono for two weeks of filming. During that period, he had to fly to Venice, London, New York, and Los Angeles for premiers of The Black Dahlia.
The little girl vampire has arm tattoos that are visible for a few frames. The stick figure-like tattoo on her right arm is the logo of German industrial band Einstürzende Neubauten.
The trivia items below may give away important plot points.
The Stranger and the grandmother were killed in the original script. The scenes where Eben and Stella find The Stranger in the station, and when Marlow comes back to him, were written during production and squeezed into the shooting schedule with the understanding that they would probably not be used.
This film is not the first time Mark Boone Junior has played a vampire killer who meets his end at the hands of the head vampire. The same thing happened in John Carpenter's Vampires.