When Langdon, Teabing and Sophie are in Teabing's house and Silas is creeping up, jumping over the wall etc; inside the house, it is obviously getting progressively lighter. Cut to Silas outside, in the pitch black.
When Fache visits Andre Vernet in the hospital, he calls him Vernet Andre at the beginning of his interrogation - putting his last name first. Then he doesn't speak his name again. The English subtitles has Fache start into his interrogation using Andre Vernet's name (used correctly this time) later on in the discussion - not at the beginning.
When Silas shoots the Bishop, there is a shot from behind Silas where he bends down. You can see the shape of the mike battery pack under just above the rope tied around his waist to help support his robes.
Sir Leigh Teabing refers to the phenomenon of peoples' minds interpreting the same thing differently ("seeing what it wants to see") as scotoma. In reality, it's called pareidolia. Scotoma is the natural "blind spot" inherent the eyes of most mammals with good vision.
A mistake occurring in both the book and the movie, Robert Langdon identifies the 5 pointed star on Sauniere's chest as a "pentacle". It is, in fact, a pentagram. The star is only called a pentacle if it is surrounded by a circle (a "penta-" "-cle").
'Leonardo da Vinci''s The Last Supper is referred to as a fresco. Leonardo painted The Last Supper on DRY and not wet plaster, so it is not a true fresco. Because a fresco cannot be modified as the artist works, Leonardo instead chose to seal the stone wall with a layer of pitch, gesso and mastic, then paint onto it with tempera. Because it is not a true fresco, it cannot be moved easily, and this fact has caused it much deterioration and damages over the years. Furthermore, as it has received so much restoration, it is impossible to read as much into the detail of the painting as the narrative implies, as the level of detail concerned is more restoration than Da Vinci's original.
When Teabing explained the situation of Rome at the time of Emperor Constantine to Sophie, he mentioned that "Three centuries earlier, a young Jew name Jesus had come along, preaching love and a single God." This statement is false, Jesus did not preach a single God. Jesus did his ministry only among the Jews in Israel, who already believe in a single God. It was Jesus' apostles, Peter and Paul, who later came to Rome and preached Jesus' gospel and a single God to the Romans who still worshiped many gods.
Teabing says that Jesus Christ was regarded as a human prophet until Constantine's party narrowly voted to deify Jesus in 323. In fact, Jesus Christ had been regarded as God in human form in most Christian documents since AD 95 and possibly early as AD 40. Around 300 a "heresy" was proposed to downgrade Jesus to a mere human prophet, but this new idea was voted down in a landslide in 323 in favor of the original concept of Jesus as God. This council was simply telling everyone what most already believed.
Langdon and others continually refer to 'Leonardo da Vinci' as "Da Vinci". However, "da Vinci" refers to his city of origin, and is not technically a surname. Historians and art aficionados always refer to him as Leonardo.
When Langdon tells Sophie about The Holy Grail, he says the story started "more than a thousand years ago", with a crusade that was started to capture Jerusalem. The crusade he is referring to, was commanded by Godfroi Du Bouillion in 1099, so it won't be "more than a thousand years ago" until the year 2100.
Langdon and others continually refer to Leonardo da Vinci as "Da Vinci". However, "da Vinci" refers to his city of origin, and is not technically a surname. Historians and art aficionados always refer to him as Leonardo.
When Sophie is being held at knife-point by Silas, you see blood trickling from the cut the knife point makes. When the scene shifts back, there is no blood or cut. When it shifts again, the cut and blood are back, and she has the cut/healing wound for the rest of the film.
When they are on Teabing's plane, Sophie goes back to Silas to question him. She rips the duct tape off his mouth. Then Langdon gets her to walk away. Later, Sophie, Langdon, and Teabing are talking at the front of the plane, and Silas can be seen in the background, with the duct tape back on. Neither Sophie nor Langdon had replaced the duct tape prior to leaving Silas.
As Langdon and Sophie walk away from the Roselyn chapel in discussion there is a distinctive 1960s light blue Morris Minor Traveller and another small car in the background. The cars then change to a black Range Rover Discovery and Rover 75 in the next take from roughly the same point. A few moments later a wide shot of the church shows just two cars in the grounds.
When the plane is taxiing on the ground in England, from the view in the cockpit it is clear that the taxiway ahead dead ends in a field with woods to the right. From the outside view the plan is shown going straight through the intersection past the signalman, then turning right into a hangar.
When Fache shows Langdon the hidden writings with the UV lamp, there are only the Fibonacci's suite and the Latin quote. Later in the bathroom, Sophie Neveu tells him Fache erased the last line which was "P.S. find Robert Langdon" and shows him a picture with the complete message. Later, when they both come back to the murder's scene, Neveu and Langdon are trying to decipher the hidden message using the UV lamp. For a short moment, over Langdon's shoulder we are able to see the line Fache has supposedly erased.
When Teabing is pointing his gun at Sophie and he wants her to open the cryptex, we see a close up of Robert Langdon who says "one moment". He has his left hand raised. In the next shot, when we see Langdon and Teabing, Langdon's hands are down.
When the plane takes off from Paris, it is pitch dark and clearly night time. When it lands in London just an hour away on similar longitude, it is broad daylight and (from the London street scenes) clearly well into daytime.
As Robert and Sophie travel in the back of the armored van to Leigh Teabing's residence, they hold hands in an attempt to calm Robert due to his claustrophobia. As they hold hands, an intermittent yellow light from an external source (supposedly street lamps) is seen. It would be impossible to see any such light from within the back of an armored van. It has also been revealed that they are traveling along a secluded country track with no street lighting.
Sophie presses the tracking device he's found in his pocket into a (white) bar of soap he's taken from the toilets and throws it out of the window into the back of a truck. The Louvre toilets are supplied with large lemon-shaped (and lemon-scented... and lemon-colored) soaps fixed to metal rods over the sinks. The Louvre toilets do not have windows at all.
When flying in the private jet and taking a call from Silas, the Bishop Aringarosa is using a cellular (GSM) phone. At this height, confirmed by a shot through the window revealing the clouds canopy below, it is impossible to get mobile network coverage.
Vinegar or scientifically known as acetic acid is a weak acid and thus unable to dissolve a sturdy material such as papyrus. Even a strong acid, like hydrochloride acid, will takes hours to dissolve papyrus. So actually they can just force open the cryptex and the vinegar-soaked papyrus would still be intact and readable.
Teabing says that Constantine converted to Christianity on his deathbed. While it is true that Constantine became a follower of Christianity much earlier, it wasn't until he knew he was dying that he was baptized by the Arian priest Usebius. This was in accordance with the custom that a person was not baptized until old age in order to absolve them from as many sins as possible. Constantine died a few days after his baptism. This would technically make Teabing's remark true by today's standards, which call for converts to be baptized into the church. In today's world, Constantine was not a true Christian until he was baptized.
The cryptex was said to be made by 'Leonardo da Vinci', and the clue to open it refers to Issac Newton's grave. Da Vinci (1452-1519) died 124 years before Newton (1643-1727) was even born, however Robert Langdon says that the cryptex was only designed by da Vinci, not made by him. In the novel, Sophie states that this cryptex was built by her grandfather.
In Langdon's opening presentation on symbology, he shows a series of slides of modern symbols and their ancient origins. The CND "peace sign" logo is shown followed by an inverted crucifix. In fact the CND logo was created in the 1950s in Britain, by superimposing the two semaphore symbols for "N" and "D", to stand for "nuclear disarmament". The false "broken cross" history of the symbol was invented in the 1970s in the United States - suggesting Langdon didn't do his research properly. However, Langdon's presentation doesn't just speak of the intended meaning of symbols, but also of the (often presumptuous) interpretation of them, making the CND slide particularly relevant.
The cut of the initial sequence of the movie (intercuts between the Louvre murder and Langdon's lecture), as well as the fact that the police officers come for Langdon during his post-lecture signing of books, create a major continuity lapse in the police' reasoning that Langdon is suspect. How could he commit a murder in Louvre (which can be easily timed due to the victim activating the alarm) while giving a lecture to thousand people? (In the book, he is visited by police several hours after the lecture, which would then allow him enough time to possibly commit the crime, as he is suspected.)
One of the key "facts" used to justify the alternative interpretation of the Holy Grail is that there are no cups or glasses on the table in 'Leonardo da Vinci''s Last Supper. In actual fact there is one immediately next to Jesus' left hand, which can be seen in high definition reproductions of the original.
In the scene where Leigh Teabing is holding the gun to Sophie's head in Westminster Cathederal, the hammer is cocked. But when Sophie picks up the gun after Teabing drops it to try to catch the cryptex, the hammer is down (yet no shot was fired).
As they are discovering Magdalena's crypt, Langdon pulls a carpet away to reveal a Fleur de lis. As the carpet is pulled away it is quite clean, however when the camera closes in it is covered in dirt and dust.
Right after Silas turns and shoots at Aringarosa he goes up to him. The pistol's slide is open (gun empty)/closed (gun loaded) between shots. Then when he turns to the police, the slide is closed again.
When the cryptex hits the floor and the vinegar vial breaks, it sprays some of its content and keeps rolling and is then manipulated by Teabing. After Teabing is being arrested you see a close-up of the cryptex on the floor and it's lying in a very large pool of vinegar - too much content for the little vial and too much regarding the fact that some of it has already been spilled.
Aringarosa is shot by Silas on a lane way which has a gradient. A camera angle shows an aerial view of the body. A pool of blood appears to surround the upper body and an area above his head. This could only happen if it could flow uphill. However, as is clear from earlier close-ups, this is not a pool of blood but his cape.
The film suggests that Sophie Niveau is descended from Christ and that this can be proven by comparing her DNA to that of the body of Mary Magdalene. Even if this were done and a DNA match confirmed it would only prove she was related to Mary Magdalene not Christ.
When Robert and Sophie go to the Roslin church and go down to the lower chamber, Mary's Sarcophagus was supposed to have been there at one time, yet it would have been impossible to get the huge Sarcophagus down there through the narrow passage, unless the church was built around it. If that is true, then how was the Sarcophagus removed from the cellar of the church?
Neveu is said to be the *only* living descendant from Christ and the Merovingian Royal Family. However, Prince Charles, Juan Carlos de Borbón, Kronprinsessan Victoria and virtually all other living royal family members of Europe, are almost certainly descendants of of the Merovingians, and therefore according to the movie, descendants of Christ.