Perhaps the most radical departure from the book is the timeline; the book starts in the summer of 1995 (as established in Chamber of Secrets), but when Harry carries Dudley home a 2006 numberplate is clearly visible in the background, and shortly afterward when Harry travels to London we see the London Eye (not erected until late 1999). Clearly the makers of the movie wanted to set it closer to our time, and J K Rowling didn't object to this.
As with many of the movies, there is a longer sequence in the book at the Dursleys, who receive multiple owl messages the night of the dementor attack. When the Dursleys want to expel Harry from their house, one of the magic letters, in an unknown booming voice, reminds Aunt Petulia of a 'promise' she once made; she reluctantly convinces Vernon to let Harry stay. She gives no explanation, but she obviously knows more about wizarding than she has previously let on. The voice is later revealed or hinted as Dumbledore, as the letter that was left with Harry when he was a baby was said to explain that Harry could not be hurt by Lord Voldemort as long as he has a home with blood relatives until he is the wizarding adult age of 17.
Harry's overall attitude has been strongly toned down in the movie. In the book, he bursts into several fits of rage directed at his friends when he feels frustrated, grieved or misunderstood. The darker sides of Harry's character and the implied similarities he shares with Voldemort are less overt in the movies than they are in the books (although the book implies that Harry's rage is largely fueled by Voldemort's anger, through their mental connection).
Much more time is spent at Grimmauld Place. The character of Mundungus Fletcher, a shady small-time criminal member of the Order of the Phoenix, is introduced (perhaps he is one of the silent Order members accompanying Harry to Grimmauld Place in the movie, but he has no credited appearance in the film). There is constant turmoil coming from the painting of Sirius' late mother Walburga, who was a silent supporter of Voldemort, and therefore firmly opposed to the presence of the Order in her house. Kreacher is still very loyal to her, and executes Sirius' orders with the utmost reluctance (while constantly muttering vile curses under his breath, seemingly unaware that everyone can still hear him). In Harry's room at Grimmauld Place, there is a painting of Phineas Nigellus Black (the least popular Headmaster of Hogwarts, according to Sirius), who also has a painting in Dumbledore's office at Hogwarts, so that his image can travel back and forth between the two places. There is a memorable scene where Mrs. Weasley encounters a boggart, which shows her greatest fears to be her family and Harry dying horrible deaths. Ron and Hermione are notified that they will be prefects for the new school year, but Harry won't (which is cut from the film). Also at Grimmauld Place, the students help Mrs. Weasley with cleaning to prepare it as the headquarters of the Order of the Phoenix. One of the items found during the cleaning is a locket that becomes important further along in the series. Harry also receives a two-way mirror from his godfather, through which he can see Sirius.
Harry notices the Thestrals pulling the chariots right when he arrives back at Hogwarts, but the explanation about why he and Luna can see them and the rest can't is given much later, by Hagrid (not Luna) during a class about the animals (see below). In the book, Neville can see the Thestrals as well, because he saw his grandfather die (the movie does not suggest that Neville can see them).
There is a sequence, completely omitted from the movie, set at St. Mungo's Hospital where Harry and the Weasleys go to visit the injured Mr. Weasley. They accidentally encounter Gilderoy Lockhart, who is still recovering from his self inflicted memory charm from Harry Potter and the Chamber of Secrets. While in his ward, they encounter Neville Longbottom and his grandmother, who are visiting Neville's parents (they were once tortured into madness by Bellatrix Lestrange). Due to this omission, Harry and the Weasley's Christmas was moved to Grimmauld Place in the film.
In the movie, Neville reveal to Harry that Bellatrix Lestrange was one of the death eaters who tortured his parents. In the Goblet of Fire, the penseive reveals this to Harry after Karkaroff names Barty Crouch Jr in the scene. So Harry would already know this before Neville told him.
While at Hogwarts, Harry keeps in close contact with Sirius through owl messages and secret Floo Network calls (Sirius is nearly caught by Umbridge at one such instant, causing her to start monitoring the Network). Sirius, being still a fugitive, needs to stay in Grimmauld Place at all times (since it was implied that Voldemort's Death Eaters now know he is an unregistered animagus due to Wormtail informing them), causing him to become edgy and irritated, and he frequently clashes with Severus Snape, whose role in the Order becomes ever more prominent. Harry hears about Dumbledore's wish for him to learn Occlumency from Snape during the Christmass holiday spent at Grimmauld Place (in the movie, he is taken for his first lesson immediately after the attack on Mr Weasley). Upon hearing this, Sirius threatens Snape with serious consequences (almost to the point of duelling), should Snape abuse these lessons to harass Harry.
When inquiring about a suitable place to train with Dumbledore's Army, Harry learns about the Room of Requirement from Dobby the House Elf. In the film, Neville is the one who reveals the Room of Requirement instead due to Dobby's omission. Dobby is also the one to warn the army when Umbridge raids the place.
A long plot-line in the book, completely omitted in the film, concerns Quidditch. Harry's detentions get in the way of practice, Ron joins the team as Keeper, and the team is nearly disbanded after an Educational Decree. Draco taunts Harry at one instant, causing Harry to punch him. Professor Umbridge reponds to this by banning Harry from playing Quidditch for life (along with Fred and George Weasley). Ginny joins as Seeker after Harry's been banned, but Ron's insecurity about his Quidditch talents gives the team little chance to win. This is worsened by the Slytherins, who keep taunting Ron with their chants during the matches.
Dumbledore appoints Firenze, a Centaur first seen in Harry Potter and the Sorcerer's Stone, as Professor Trelawney's replacement for Divination. This angers the rest of the Centaurs (who feel that Centaurs should not mingle in Wizard affairs) and explains their aggressive behavior towards Harry, Hermione and Umbridge later (along with the fact that Hagrid's half-brother Grawp has been disturbing their habitat). The film omits this and instead implies through dialogue (and a Daily Prophet headline created for the film) that the Ministry had been angering the Centaurs by restricting their territory.
Most of the awkward romance between Harry and Cho has been omitted, including a disastrous Valentine's coffee-shop visit (Harry tells Cho about an appointment he has with Hermione later that day, which Cho mistakes for a date, so she rushes off in tears). In an attempt to get the truth about Voldemort out, Harry gives an exclusive interview to Rita Skeeter, telling details of Cedric Diggory's death. (In Goblet of Fire, Skeeter had been blackmailed into silence by Hermione, who discovered she was an unregistered animagus). The interview is published by Luna Lovegood's father in his magazine, The Quibbler.
The book has the Inquisitorial Squad in a much more prominent position. There are numerous instances of Draco Malfoy and other members abusing their powers to scare, bully and remove House Cup points from various students. After Professor Dumbledore escapes arrest, Professor Umbridge finds that she cannot enter the Headmaster's office, as it simply refuses to recognise her as the new Headmistress.
In the book, Dumbledore's Army is not betrayed by Cho Chang (who was coerced with Veritaserum in the movie), but by another girl named Marietta, who suffers a horrible case of acne because of a hex Hermione had placed on the sign-up sheet. Note that the other girl was invited by Cho to the army's meetings, so Cho felt guilt in any event. She tries to apologize to Harry, but defends Marietta's reason, which was to protect her mother's job at the Ministry, due to Fudge's threats of firing anyone from the Ministry that are discovered to have sided with Dumbledore. This enrages Harry, and the ensuing argument ends their relationship prematurely.
The way in which Harry gets to see Snape's worst memory is different. In the movie he reverses one of Snape's Legilimency spells; in the book, Snape empties some memories in Dumbledore's Pensieve every time before the Occlumency lesson begins, in order to prevent Harry from inadvertently seeing them. At one such lesson, Snape is called away by Draco and the Inquisitorial Squad, and Harry uses this moment to look into the Pensieve. The flashback is more detailed. Sirius Black, Remus Lupin, and Peter Pettigrew were present when James Potter harrassed Snape, and Harry's mother intervenes to stop them from bullying him. However, an embarrassed and bitter Snape calls Lily "Mudblood" for being friends with James. At that point, Harry is pulled out of the Pensieve by Snape, who furiously dismisses him.
There is much more about the O.W.L.s and how the students do on them. The students are told they have to choose a career path in order to determine which classes they need to take. Harry decides he'd like to be an Auror, which causes an argument between Umbridge and McGonagall: Umbridge points out that Harry has a criminal record (underage magic in presence of a Muggle), and his grades for Defence Against The Dark Arts are insufficient; McGonagall retorts that Harry has been exonorated by the Ministry of Magic, and his grades were fine before Umbridge became professor. During one of Harry's O.W.L.s, he gets to perform a Patronus for an extra point.
There are two separate instances of disruptions made by the Weasley twins, the second intended to help Harry use Umbridge's fireplace for communication with the Order (due to it being the only fireplace in Hogwarts not being monitored). In the first instance they use their own brand of fireworks and in the second one, they conjure a swamp in one of the corridors. Harry manages to use Umbridge's fireplace, and shortly talks with Sirius and Lupin about his father, and the distressing situation he witnessed in Snape's memory. They assure Harry that his dad became a much better person, and that Snape wasn't as innocent as he may have appeared. Unfortunately, the twins are caugt. They publicly take credit for the swamp, to the point of advertising their Portable Swamp, and leave the school and are thus never actually expelled. In the film they leave after releasing their firework, and the communication to Sirius and Lupin is omitted.
In the scene where Fred and George leave Hogwarts, before they leave, they tell Peeves (a nasty troublemaker of a poltergeist) to "Give her hell from us!", referring to Umbridge. There is also more in the book about Peeves, amongst other things, for the first time ever working together with the students. Also, McGonagall seems to favor Peeves' mischief (since she also dislikes Umbridge): as she walks by Peeves, who is trying to unscrew a chandelier, she mutters, "It unscrews the other way." There is also a running theme in the book omitted from the movie about Fred and George starting a joke shop using the winnings that Harry gave them from the Triwizards tournament in The Goblet of Fire. They had been perfecting their craft and dropped out of school to follow their dream with a legendary exit.
In Hagrid's absence, his 'Care of Magical Creatures' lessons are taken over by Professor Grubbly-Plank, who makes the course more structured and less dangerous than Hagrid. Many of the students like the new teacher and lessons better, but Harry, Ron and Hermione are conflicted because of their friendship. When Hagrid returns in the film, his return to teaching is not shown. In the book he tries to hold a class about Thestrals while being much interrupted and disturbed by Dolores Umbridge during the review. Hermione encourages him the teach less risky lessons and helps him with lesson plans so that he can get a positive review. Before that, he introduces Harry and Hermione to Grawp, who is much more aggressive and less good-natured than portrayed in the movie. In the book, Ron does not meet Grawp when Harry and Hermione do. He is off playing in the Quidditch final, in which Griffindor unexpectedly wins, and Harry and Hermione tell him about Grawp much later. Hagrid is later fired by professor Umbridge, and even faces arrest by the Ministry. Because of an intervention by Professor McGonagall, he escapes. However, McGonagall is severely injured by stunning spells during this altercation, ending up at the hospital wing.
In the book, Harry gets his vision of Sirius being tortured by Voldemort in the middle of an O.W.L. and not after Fred and George's final stunt. He faints and is dragged away. His friends try to convince him that it may be a fake vision by Voldemort, but he does not want to hear of it. They set up another diversion in order to lure Umbridge away, so they can use the fireplace in her office. Harry manages to make a 'call' to Grimmaulds Place, where Kreacher tells him Sirius has indeed left the house, but Harry is abruptly interrupted by Umbridge and the Inquisitorial Squad. Dolores Umbridge herself reveals to be the one who sent the Dementors after Harry and Dudley (see FAQ above).
The sequence in the Department of Mysteries is much longer. There are many rooms within the department, one of them being the circular room with the stone archway. One room contains a large tank with brains floating inside it, another has a large bell jar with a hummingbird inside, which is reborn over and over again. The members of Dumbledore's Army and Death Eaters battle and hex each other all through the different rooms of the Department. The fight is drastically altered in the film, where the DA members aside from Harry are captured by the Death Eaters to force Harry to turn over the prophecy; in the book, Harry ends up in the Death Chamber when the others take various injuries, and some of them are rendered unconscious. Neville (whose nose is broken) is tortured by Bellatrix to force Harry to give over the Prophecy, but the arrival of the Order of the Phoenix prevents this. While the two groups are fighting in the Death Chamber, it is Neville (not Lucius), who drops the prophecy by accident in the book; nobody hears what it contained (in the movie, Harry can hear it when he holds it). Sirius Black is not killed by the killing curse in the book. It does not define which curse is used, except to say that there were red lights/sparks (the killing curse is known for having a green light). Sirius is stunned and then falls over, but he tragically tumbles through the arch, which seems to be a one-way passage to death. The assumption is that Avada Kadavera was used in the movie for people who do not read the book, thus saying with certainty that Sirius Black is dead.
When Harry, Bellatrix and Voldemort are in the lobby of the Department of Mysteries, Voldemort is furious to learn that the prophecy he has been after for so long has been smashed, and he attempts to kill Harry using the Avada Kedavra curse. Before the spell can reach him, Dumbledore summons the golden statues in the lobby's fountain to protect Harry and capture Bellatrix.
Harry's attempted possession by Voldemort is made much more dramatic in the film, whereas in the book Harry isn't sure what's happening to him and is informed after the fact by Dumbledore what Voldemort was attempting to do. None of the dialogue of Voldemort speaking through Harry or Harry rejecting Voldemort is in the book. When the Aurors arrive, Voldemort grabs the incapacitated Bellatrix and Disapparates.
Harry is also much more upset after Sirius' death, to the point of angrily breaking objects in Dumbledore's office. It should be noted, though, that in the book, Harry is immediately transported to Hogwarts after the fight at the Ministry, when his rage and grief are still fresh. In the movie, there seem to be several days in between, so Harry had some time to cool off and come to terms with Sirius' death.
It is revealed that The Order of the Phoenix was betrayed by Kreacher, Sirius Black's house elf, when Sirius, in a fit of anger, gave Kreacher an order that permitted him to speak to Sirius' cousin, Narcissa Malfoy. On her suggestion, Kreacher had lied to Harry on purpose, by saying Sirius had left the house, in order to lure Harry to the Ministry. Harry voices his suspicion of Snape, thinking he may have tricked Sirius towards his death, and used the Occlumensy lessons to open Harry's mind to Voldemort, but Dumbledore quickly dismisses these thoughts, stating he has complete faith in Snape's loyalty; he simply underestimated that Snape's resentment towards Harry would get in the way of their Occlumensy lessons. Dumbledore also subtly implies that Sirius may have warranted Kreacher's betrayal, and thereby caused his own death, by treating Kreacher so badly, which enrages Harry even further.
The explanation Dumbledore gives to Harry about all events of the past year is much more elaborate. Firstly, the reason why Harry was left with his condescending aunt and uncle, and why he was sent back there every summer: when Harry's mother was killed trying to save Harry, her sacrifice out of love created a magic protection for him (the 'old magic' Voldemort referred to in Harry Potter and the Goblet of Fire). The proximity of her blood protects Harry from harm until he comes of age. As Aunt Petunia, being Lily's sister, carries the same blood, the protection also works in her presence, as long as Harry is underage. So the place Harry always detested most is, ironically, the safest place for him to be. Dumbledore explained this to Petunia in the letter he left on baby Harry, and made her promise to keep him there for his protection. The booming voice coming from the letter in the beginning, reminding Petunia of her promise, was Dumbledore's.
Also, it is explained that Dumbledore knows about what was said in the prophecy because he heard it predicted years ago by Professor Trelawny, who came to apply for the job of Divination Professor. This is why she got offered her job. Since no one heard the Lost Prophecy in the Hall of Prophecies, Dumbledore shows Harry his memory of it in the Pensieve. It turns out that the prophecy spoke about a boy who was soon to be born from a wizard family, meaning it could have applied to either Neville Longbottom or Harry Potter. Dumbledore believes Voldemort saw Harry as the one to whom the prophecy specifically referred, because they are both half-blood, and he therefore felt some sort of connecting bond. Dumbledore also explains that a Death Eater (revealed in the book as Snape) overheard the beginning of the prophecy, but missed the crucial part about the Dark Lord 'marking his nemesis as his equal', which caused Voldemort to carelessly attack Harry and his parents (and by trying to prevent the prophecy from happening, he created his nemesis, and inadvertently fulfilled the prophecy himself). The 'power which the Dark Lord knows not', is love, according to Dumbledore; it is his mother's love which saved Harry from death, and it is the one thing that Voldemort lacks completely. Harry's love for his friends is what actually caused Voldemort's inability to completely possess Harry at the Ministry.
Harry finds Ron and Hermione in the hospital wing, as well as an almost catatonic Umbridge, who was saved from the Centaurs by Dumbledore after he returned to Hogwarts. Harry knows he cannot tell Ron and Hermione about the prophecy. On his way to Hagrid, he is threatened by Draco for getting his father arrested. Harry is ready to defend himself, but Snape intervenes and once again, tries to deduct House Points from Gryffindor (they have none left, though); however, Professor McGonagall, just released from the hospital, bestows Gryffindor 200 points for letting the wizarding world know of Voldemort's return. Harry decides to skip the final feast of the year, and while packing his trunk, he finds Sirius' mirror again. Harry takes a peek in this two-way mirror, hoping for a sign of Sirius from beyond, but it remains empty. Realizing that it is useless, since Sirius did not have the other mirror with him when he died, Harry angrily breaks it. Harry has a conversation with Nearly Headless Nick, broaching the possibility of Sirius returning as a ghost. Nick explains that he and most other ghosts chose their fate because they were afraid of leaving this world. Harry finds Luna in the hall, who explains why she can see Thestrals as well (explained in the movie very early on).
Draco, Crabbe, and Goyle confront Harry at Hogwarts and give him death threats. His friends from Dumbledore's Army jinx them before they can hurt Harry. Harry tells them that after confronting Voldemort and the Death Eaters, he's not afraid of anything they might try. At King's Cross Station, Professor Moody warns the Dursleys to give Harry a proper treatment this time, or he will intervene.