At the beginning of the movie, Chicken Little says that the piece of the sky was shaped like a stop sign. Later, the panel only has six sides, and he still says it's shaped like a stop sign. Stop signs have eight sides.
When Chicken Little rings the bell for the Aliens, his father is watching the weather on TV. The forecaster says, "There's a cold front moving towards us" but all the fronts indicated on the map are red, warm fronts. (This is a meteorological standard: red half-circles for warm fronts, blue triangles for cold.)
When the panel falls out at the end, Melvin's wife pushes the button and sends the panel into the middle of the ship's underside. When Fish's glow-stick is spinning and the ship comes to a rest, the panel is the one from the outside edge.
When the UFO landed in the ballpark with fish stuck inside, you can see the green glow stick stuck on the bottom part of the UFO. The next scene you cannot see the glow stick or the green light coming from the glow stick. After that scene the glow stick is back.
During the baseball game, Chicken Little hits the ball and it gets stuck on the right horn of the bull in center field. Once Chicken Little gets to home plate and the bull taps Chicken Little with the ball, it is on the bull's left horn.
When Chicken Little is called into the baseball game, the cat character sitting next to his father, Buck Cluck, is identified in the subtitles as a Cheetah and yet has no spots. Later on in the movie, he does appear with spots.
When Chicken Little is running after the school bus, Foxy dumps out a bag of acorns to make him trip. When Chicken Little falls, the acorns are all around him. However, when he gets back up, the acorns are gone.
When Chicken Little, his father, and Kirby run up the stairs to the top of the town hall building so that Kirby can be given back to his parents, they pass a sign in the stairwell indicating 10th floor. But when they entered the building, the out of service elevator indicated that there were only 5 floors. From the exterior of the building, it is also clear that there are not more than 5 stories.
At the end of the movie they all watch "Chicken Little - The Real Movie" and when it ends the father lifts up Chicken Little. Afterwards you can see that the seat where Chicken Little sat before has disappeared.
When Chicken Little and his Buck Cluck have climbed to the top of the Town Hall, and after Buck has knocked out all the Aliens that were around them, Chicken Little is holding Kirby. But, after they are zapped by the spaceship above them, when they reappear inside the spaceship, Buck is holding Kirby not Chicken Little.
Chicken Little loses his pants on the way to school and has to wear makeshift paper pants, which are ruined when they get drenched in gym class. Yet when he is in the principal's office in the next scene, his real pants are mysteriously back.
Pulling the fire alarm does NOT activate a sprinkler system; it only sounds the alarms. However, many sprinkler systems are designed so that when a sprinkler is set off, it triggers the fire alarm system in turn, not the other way around.
When Chicken Little gets his big hit in the baseball game, by the time he heads for home, the game is tied. If he is called out, it wouldn't be the big tragedy it's played out to be, they would still have a chance to win in later innings.