Jessica Biel inadvertently destroyed a camera, costing more than $300,000, when she fired an arrow directly into the camera's lens. She was directed to "aim for the camera", which had a Plexiglas shield in front of it to protect it, except for a small opening in front of the lens. Biel had perfected her archery skills while training for this role to such a degree that when she fired the arrow - at a distance of approximately 50 feet - at the camera, as she was directed, it went directly through the lens and into the camera itself, destroying it. The footage of the incident is included in the DVD extras.
An early idea by David S. Goyer for the film was to be set many years after the events of Blade II (2002), where vampires finally had achieved world domination and enslaved all humans, with Blade being the last hope for humanity. Blade's slower aging could be explained by his vampire blood. The storyline was deemed too dark and was later dropped.
Wesley Snipes and 'Kris Kristofferson', who at the time had become good friends after working on the two previous Blade-installments, were reportedly unhappy with this movie and with David S. Goyer's script decisions. They felt that too many new characters were added to the universe, and that Blade did not need any sidekicks besides Whistler.
There was much on set drama between Wesley Snipes and director David S. Goyer in regards to certain decisions made with the film. This is evident in the making of featurettes as Wesley Snipes has little to no participation.
An early idea of David S. Goyer was to include not only Hannibal King, but a female character called Rachel Van Helsing from the Tomb of Dracula comics, but then he heard about the movie Van Helsing (2004) and decided against it. He ended up creating the character of Abigail Whistler, Whistler's daughter, in her place.
The car Blade drives in all three movies is a 1968 Dodge Charger modified with UV lights behind the front grille as well as numerous switches inside the car. After the movie the car was purchased by a man in Vancouver who kept the car virtually the same except for adding a 440 under the hood.
The vampire "final Solution" in this film originally came from an idea in Blade (1998), the first film in the trilogy. In a deleted scene Deacon Frost shows Karen Jensen a prototype of harvesting human bodies. This can be found on the "Blade" DVD.
When Hannibal King is telling Blade about the return of Dracula, he shows Blade a copy of Tomb of Dracula #55. Marvel's "Tomb of Dracula" comic was the early-1970s title which included the first appearances of Blade (#10) and Hannibal King (#25), both written by Marv Wolfman.
The film crew was forced to be selective in their shots for various green-screen segments because neighboring stages were being used for the filming of I, Robot (2004) and The Chronicles of Riddick (2004) and those sets would sometimes appear within the frames (and edited out later).
After the car crash that ends the opening chase scene, one of the benches at the bus stop in the background displays a poster with the word "esperanto" on it. Abraham Whistler (Kris Kristofferson) and the newspaper vendor are speaking in Esperanto, with English subtitles provided. The scene on and in the police headquarters is in both English and Esperanto. The movie watched by Hannibal King when he is recovering from his wounds on the boat is Incubus (1966) with William Shatner, which was shot in Esperanto. The Esperanto League for North America was contacted and asked to provide the necessary translations for the movie.
Wesley Snipes has less than a hundred sentences in this movie, including one-word sentences (which make up a majority of Blade's lines) and even onomatopoeic sentences, like Blade's "goochie goo" to the baby he saves.
Series Trademark: [sunglasses] The sunglasses worn by Abigail Whistler (Jessica Biel) are Oakley Half-wires. The brand made many appearances in the trilogy, mostly in Blade II (2002). This has been attributed to Wesley Snipes' love of the brand. Oakley sunglasses also make regular appearances in the Blade: The Series (2006).
The opening scene with the helicopters in the Syrian desert was originally scripted to be in Iraq. However, consultants pointed out that, at the time, unmarked helicopters would have either been shot down by the U.S. military or shot at by Iraqi insurgents. Goyer changed the scene to Syria.
According to Goyer on the commentary, he says that 'Christop Heyerdahl' offered to speak with a Norwegian accent. Goyer consented but stipulated that Heyerdahl maintain the accent throughout production. Heyerdahl was said to be convincing enough, that Snipes even believed Heyerdahl was Norwegian. Coincidentally, Heyerdahl would later play "The Swede" on Hell on Wheels with a Norwegian accent.
The trivia items below may give away important plot points.
When Blade and Whistler return to find Sommerfield dead, the phrase "Immortality will come to such as are fit for it" is painted in blood on the shower curtain. This is a quote by Ralph Waldo Emerson. The full quote reads: "Immortality will come to such as are fit for it; and he who would be a great soul in the future must be a great soul now."