According to writer/actor Kevin Grevioux, the model for the Selene character was the psychic vampire of the Hellfire Club in the X-Men comics, the Black Queen Selene.
Len Wiseman opted to shoot many of the action and effects sequences live, without computer imagery. In one scene, for instance, a werewolf seems to run more than 50 mph behind a speeding car. This was shot using an elaborate rig towed behind a vehicle, with actor Michael Sheen doing the closeups of the running and the jump onto the car, while the long shot of Lucian running was done by stuntman Todd Schneider.
White Wolf, Inc. and author Nancy A. Collins filed suit against Sony Pictures, Screen Gems, and Lakeshore Entertainment on 4 September 2003 for copyright infringement little more than a week before the theatrical release of Underworld, alleging 17 counts of copyright infringement, and claiming over seventy points of unique similarity between White Wolf's role-playing games, "Vampire: The Masquerade", "Werewolf: The Apocalypse", and their creation, "The World of Darkness", in which the games are set. Nancy A. Collins is the author of a short story, "The Love of Monsters", published in 1994 by White Wolf Inc, and set in the World of Darkness, which she claims the entire plot of Underworld is based on. The suit was settled out of court, for an undisclosed amount.
Days before its US opening, the president of Screen Gems green lit not only a sequel to the movie Underworld but also a prequel after seeing the number of tickets sold in Canada alone.
The ancient tome which Selene uses to research the history of Kraven features pages from three different sources, all shown before "The Fall of Lucian." The first, featured on the page with the seals of Amelia, Viktor, and Marcus, is an ancient Hungarian text entitled Funeral Sermon and Prayer. The second is an old Catholic Roman Missal, including the Propers of the Second Mass for All Souls' Day. The third is a book entitled Instructissima Bibliotheca Manualis Concionatoria, which is also featured between pages of the story of the fall of Lucian.
The 'attack dogs' outside of the vampire mansion were actually very docile and playful canines, which were the only dogs available at the time. For the scene where they chase Scott Speedman, director Len Wiseman had to film short clips of the dogs running and later put in sounds of vicious barking. If you look closely though you can see their tails happily wagging back and forth.
The name of the city where the film took place is never mentioned. However Michael's address that we can see on Selene's computer is an apparently misspelled Hungarian address (it should be "Lakatos József u. 39." while it is spelled as "Laktos Joszef 39 ut." instead). You can also read "Eötvös utca", a street in the historical downtown of Budapest, many times while driving by to the secret interrogation place and there is an ad in the subway station where the shooting takes place in the beginning of the movie referring to Szentendre which is a small town near Budapest (obviously left there as non-Hungarian viewers don't understand the text anyway).
The underground tunnels used for the Lycan/vampire battle at the end of the movie were mostly shots of the same small rubble set, but shot from different angles and lighting in order to make it look like different locations underground. During the DVD commentary, director Len Wiseman laughed at this fact and pointed out the scenes in which this trick photography was used.
A kiss between Michael and Selene (when she unchains him in Lucian's lab) appears in the trailer but was not included in the theatrical release or on the DVD, but it is on the unrated version of Underworld.
Scott Speedman suffered a concussion when a set prop that was supposed to be a piece of the wall Viktor had just thrown him through hit him in the head.
Kate Beckinsale's character Selene is not seen speaking until roughly 12 minutes into the film, however her narration starts almost as soon as the movie does.