In the opening sequence Kimberly's car keys are shown. Her key chain is an evil eye bead. The evil eye, in middle eastern cultures is a talisman that wards off evil spirits and negative energy, and by doing so protects the owner. There are also other superstitions regarding the evil eye, the main one states that the evil eye can not be bought for oneself, it has to be gifted to them.
In the movie, Ali Larter's character is at the Stonybrook Mental Institution. However, there is a real town named Stony Brook on Long Island that is home to a university and medical hospital that bears its name. The map displayed on mapquest in the movie is the actual location.
The police officer radios in an accident at mile marker 180 (the accident where the news van eventually blows up). Also when Kim is standing next to her SUV at the exit ramp just before the officer pulls her out of the way of a truck, she sees a road work sign "next 180 feet". These are references to flight 180 in the first movie.
In the original Final Destination (2000), many characters are named after black-and-white horror film stars or directors. In this movie, Kimberly Corman is named for famous horror director Roger Corman.
When the principal character enters the sanitarium the words "Get them off me" can be heard coming from one of the cells. This is a reference to a lunatic (also imprisoned in an asylum) in Hellbound: Hellraiser II (1988), who carves himself up with a straight-razor because he suffers from a delusion that he is covered with bugs.
The TV news van at the farm has the call letters WWED which is registered to a classical music station in Virginia meaning the crew picked those letters on purpose. They stand for "When Will Everyone Die"
When Officer Thomas Burke sees the pile-up and collisions, he runs to his car and radios in, "This is Unit 13, requesting medical assistance and back-up for a major traffic accident." It hardly needs to be pointed out that 13 is considered an unlucky number.
The trivia items below may give away important plot points.
Originally, Devon Sawa was to reprise his role of Alex Browning in this film, but a dispute concerning his contract with New Line Cinema could not be settled. In this film, it is implied by a newspaper clipping that his character Alex was killed off by a falling brick to the head.
When Evan is in his kitchen before his death, he leans over to get something out of his fridge and you can see the letter magnets "HEY E" on the side of the fridge. When Evan puts the leftover Chinese food container on the counter, the "H" magnet falls off and lands in the container. The letters left spell out the word "EYE," which foreshadows that Evan will die when he is impaled through the eye with a fire escape ladder.
The mortician says that only new life can break death's cycle, a life that wasn't meant to be. This is already proven false as Alex was technically dead after he was electrocuted in the first film yet he and Clear still die.
In Final Destination 2 Clear Rivers is killed by a fire in the hospital. In the first film Alex's premonition is that she is burnt on the plane, the exact same way she burns in real life on the second film.