The first 30 minutes of the film has a background noise with a frequency of 28 Hz (low frequency, almost inaudible), similar to the noise produced by an earthquake. In humans, it causes nausea, sickness and vertigo. It was a cause of people walking out of the theaters during the first part of the film. In fact, it was added with the purpose of getting this reaction.
During the party scene shortly after (or before) the rape, when Marcus (Vincent Cassel) is asked his name, he replies "Vincent" instead of his character's name. He quickly covers this up by saying "just kidding" so that they would not have to reset the long and complicated shot.
During the rape scene, a man in the background walks in the tunnel and then walks out. This was an accident as an assistant on the film accidentally walked in and walked out when he realized they were shooting. The director kept this in because he wanted to show that not everyone prevents crimes.
The "Rectum" is in fact a genuine gay S&M club in Paris, called Club Banque ("Club Bank") and located in a former bank. The crew changed the name, redressed the set and added red lighting. The club was spread across the basements of three separate buildings and was so cavernous and confusing that many of the crew members became claustrophobic in it.
This began life as a study of a committed relationship for director Gaspar Noé. He wanted to make the film with a real-life couple and husband and wife Vincent Cassel and Monica Bellucci ideally fit the bill. However, as the trio started workshopping ideas for the film, it started to take on a much darker hue.
In "An Experiment with Time", which Alex is reading during the last (i.e. chronologically first) sequence in the film, J.W. Dunne postulates the existence of a "time-travelling observer", which in dreams can move backwards or forwards in time to actually observe events which may not have yet happened. These are the 'premonitory dreams' which Alex mentions to Marcus and Pierre. Alex earlier describes such a dream to Marcus, where she is in a 'red tunnel' which breaks in two.
Monica Bellucci and Jo Prestia completed six takes of the rape scene over two nights. Bellucci claimed the first take was actually the easiest to do, since on subsequent takes she had a heightened awareness of what was going to happen and had to prevent this from interfering with her characterization.
Gaspar Noé admitted to taking cocaine while filming the scenes in Rectum, because he was so tired from operating the camera. When he was finished and the high wore off, he could not move his arms for the rest of the day.
The entire film was shot on Super 16, telecined to high-def video for color tweaking and editing, and then exported to Super 35. For many of the handheld shots, the director used the smallest existing 16mm camera, the Minima.
Anywhere from five to fifteen takes of each ten- to twenty-minute sequence were made, some as short as three minutes and some as long as fifteen. Some takes were assembled by using invisible edits disguised with digital post-processing.