Bounty Hunters Jo Junga the Aborigional, I & Am the infamous twins, The Gentleman & Boris the Russian have all tracked down Samurai Jack up North. Princess Mira of the Andaluvians convinces them to join forces and attack the Samurai together.
The Scotsman recognizes Samurai Jack working as a water boy on a boat and calling himself Brent Worthington. He then decides to find out why and where Jack got his memory erased by retracing his steps.
Princess Verbina and Prince Astor of the Lebidoptirans are on a quest to find their star navy to save their home world Chrystalis. But they are forced to crash land on Aku's Earth and are immediately captured by his minions. Luckily Samurai Jack is witness to the events and follows them.
An experimental X-series robot was given emotions as an experiment and therefore was the only one of it's kind to survive intact. It retired after taking a shine to sweet thing Lulu, but Aku forces it to take on one more job: hunt down Samurai Jack.