This marked the first animated appearances for all of the characters on the show except for Superman and Lois Lane, who had made their animated debut nearly 25 years earlier.
The Teen Titans line up was Kid Flash, Aqualad, Speedy and Wonder Girl. Notably absent was Robin. This was because Robin was appearing in the "Batman (1966)" television series at the time, broadcast by rival network ABC, so they could not use the character. Whenever the Titans contacted each other on screen at the beginning of the episode, each character would appear on separate monitors. There was one monitor that was usually blank, presumably this would have been where Robin's image would appear.
Bob Hastings voices Superboy in this series, and would later voice Commissioner Gordon in Batman: The Animated Series (1992).