Script doctor Scott Derrickson tells the story of how he got the job working on this film. He got a phone call from Harvey Weinstein, saying, "I just bought this script, called, 'Dracula 2000'." Derrickson replied, "Oh, yeah? Is it good?" "It stinks!" "So why did you buy it?" "Because it's called, 'Dracula 2000'." Derrickson did the re-write. Derrickson's rewrite was subsequently discarded by Weinstein and the script was rewritten by Ehren Kruger.
The line in the film where Jonny Lee Miller playing Simon Sheppard says, "Never, ever fuck with an antiques dealer," was a line the actor would say on set as a joke. The director liked it and incorporated it into the scene.
Alternate versions and Deleted scenes from the DVD include: An extended version of the final confrontation between Dracula and Van Helsing. An alternate version of the scene where Dracula attacks Valerie at the site of the plane crash. In this version he can be seen rising from the swamp behind her. He also says the line which could only be heard in the previews, "There are things much worse than death". A longer and much more dramatic version of the scene where Dracula talks to the Jesus on the neon cross. Dracula explains how he feels that he was the one who was betrayed. A longer version of the scene where Van Helsing and his accomplices trap Dracula in London. This was originally the opening scene to the film. A scene where vampire Val approaches Simon before leading him into the crowded streets of New Orleans during Mardi Gras. During the Judas fantasy sequence, Mary walks up to Dracula while he's hanging from a tree. The rope around his neck breaks and he attacks Mary. After finding her father, Mary walks out into the hallway where she meets vampire Lucy for the first time. This was replaced by the scene where the hallway becomes longer and Mary is approached by all three vampire brides instead of just Lucy.
The character Lucy Westerman's name is a reference to the character Lucy Westenra from Bram Stoker's book. She is in the movie, as in the book, the best friend of the main character Mina (book)/Mary (movie).