When E.L. asks for "help" from the nurse working at the sperm bank she says, "The best results seem to be obtained through fantasization accompanied by masturbation followed by ejaculation." This line was taken from a similar scene in The Right Stuff (1983), where Gordon Cooper asks for help from Nurse Murch.
Originally, Moby's version of the James Bond theme was playing in the background when E.L. drove the car over the bridge. However, Director Todd Phillips later switched it to a 'generic' song, because the budget didn't allow him to use the Moby song.
Each College in the movie, other than the University of Tennessee, is altered from names of real colleges. For example, University of Ithaca does not exist but is supposed to spoof Ithaca College and Cornell, neither does University of Austin which is a spoof of University of Texas, or University of Boston which could spoof Boston College, or Boston University
Shares a similar plot with the 1998 film, "Overnight Delivery." In both stories, a young man in a long distance relationship gets anxious after not hearing from his girlfriend in a while. He sends her a package with proof of his own infidelity and ends up chasing the package across the country to retrieve it before she opens it.
When E.L. (Seann William Scott) gets a prostate check up by the sperm bank by nurse (Marla Sucharetza), the gloves that she puts on are "Evolution One" latex gloves as indicated by the box when he enters the check up room.