Mickey's Surprise Party was edited for this release, due to the short being originally sponsored by Nabisco. All the Nabisco packaging were replaced by generic products, and all of Mickey and Minnie's lines referencing the products at the end were redubbed by Mickey and Minnie's current voice actors, Wayne Allwine and Russi Taylor.
The part in the Steamboat Willie short where Mickey uses the Mama Pig as an instrument was edited out from the video when it was released.
None of the shorts were shown with their original title cards, instead, the titles were shown on the corner of the screen, disappearing after a few seconds, with an exception for the short, Steamboat Willie.
The "Hey Mickey" song was also used on the 1996 direct-to-video release, Mickey's Greatest Hits, which also serves as the international equivalent of The Spirit of Mickey. Sound effects are also used for the song in Mickey's Greatest Hits, but are muted out in The Spirit of Mickey.
The opening music for the Steamboat Willie short was tweaked a little bit, the music started by a few minutes earlier than before.