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While on-location in Tunisia, George Lucas made one shot intended for Star Wars: Episode III - Revenge of the Sith (2005) of Obi-Wan delivering baby Luke to the Lars homestead. He claimed he would not be returning to Tunisia, and if he needed another shot, he wouldn't get it. Since Ewan McGregor did not participate in the Tunisia shoot, a wide shot of a double was filmed handing over a doll to Owen Lars (Joel Edgerton). However, during production of Star Wars: Episode III - Revenge of the Sith (2005), it was decided that Obi-Wan should hand the infant to Beru (Bonnie Piesse) instead. All three were filmed separately, in front of a greenscreen, and the original shot was ultimately not used.
When Jango Fett gets into his ship after his fight with Obi-Wan, he bangs his head on the partially open door. This was intentional, and is a reference to a famous goof from Star Wars: Episode IV - A New Hope (1977), where a stormtrooper accidentally bangs his head on a door.
The entire "aggressive negotiations" conversation during the dinner scene between Anakin and Padmé was ad-libbed by Hayden Christensen and Natalie Portman at George Lucas' request, due to his not being happy with the romantic dialogue he wrote for that scene.
Because he wanted to be able to identify himself during the colosseum scene, Samuel L. Jackson (Mace Windu) specifically asked George Lucas if he could have a purple light-saber. Lucas replied that Jedi light-sabers were only blue or green, to which Jackson said "Yeah, but I want a purple one." Lucas said he'd consider the request. Jackson says he didn't know how it would turn out until he went in for re-shoots, which is when Lucas showed him the scene containing his purple light-saber.
Hayden Christensen claimed to have greatly enjoyed filming the bar scene, because it was all a real set, and not just a greenscreen.
According to George Lucas, Obi-Wan's hiding in Geonosis' asteroid field teaches young Boba Fett a lesson that he uses to his advantage during adulthood. Having learned how Obi-Wan hid from him and his father, Boba knows the trick Han Solo is using to hide in Star Wars: Episode V - The Empire Strikes Back (1980), and is able to find him.
Anthony Daniels: As a human customer in the bar, when Anakin and Obi-Wan hunt Senator Amidala's attempted assassin, visible just after Obi-Wan draws his light-saber.
Ahmed Best: He is seen as a human customer in the bar, into which Anakin and Obi-Wan follow the assassin.