- Several shots in the sequence where Venkman, Stantz, and Spengler are thrown off campus were cut.
- Several scenes throughout the film with Janine and Egon were cut.
- The first time Venkman leaves Dana's apartment, he says to Louis "What a woman."
- The "green slimer" ghost is discovered by two newlyweds at the Hotel Sedgewick. Also cut was a Ghostbuster inspection of the room.
- A policeman tries to ticket the Ectomobile, but the car won't let him.
- Bill Murray and Dan Aykroyd play two bums that witness Louis being chased by the terror dog.
- Ray and Winston inspect Fort Detmerring, where Ray dresses in an old General's coat and falls asleep. When he awakes, he sees a female ghost above his bed. This part of the sequence was kept and used in the montage in the middle of the film.
- Louis encounters two muggers in Central Park during the ghost montage.
- Venkman and Stantz discuss matters with the mayor outside City Hall.
- The Stay-Puft Marshmallow Man sequence ends with his large hat falling to the ground. Some deleted shots appear in the film's trailers.
- A scene with Egon testing the proton pack, which is charged by being plugged in. The pack melts the plug.
- A model's mink coat comes to life on a runway. Note that this scene does happen in Ghostbusters II (1989) to a woman on the street.