To collect a $20,000 reward from wealthy industrialist Carter Stewart, Boss has crooked hypnotist Professor Crandall convince Daisy that she is Stewart's long-lost daughter, Vivian (who happens to bear an exact resemblance to Daisy). Boss doesn't count on Stewart's fortune-hunting niece and nephew wanting to kill Daisy, since they are fully aware that they are next in line to inherit Stewart's fortune on his passing.
Boss has Artie Bender, the Rembrant of Hazzard County, declared legally dead so he can sell Bender's paintings at inflated prices. It works out well for Boss, since Bender is the only one who can clear Bo and Luke of armored car robbery charges.
Luke's old girlfriend, budding country singer Candy Dix, is targeted by her abusive manager, Eddie Lee, who wants to kill her so he can collect a $100,000 insurance payment. When Luke offers to protect Candy, Eddie Lee - himself trying to run from a bookie, to whom he owes $100,000 in gambling debts - approaches Boss and Rosco to have the Dukes arrested for kidnapping.
Bo and Luke begin infiltrating J.J. Carver's organization, compiling evidence to bring Carver and his henchmen to justice. But Boss and Rosco - aware that the Duke boys have left for the NASCAR circuit but unaware they are government witnesses - nearly blow the whole operation when they confront Luke while he is secretly taking photographs of Carver's activities.
Another of Boss' corrupt nephews, Dewey Hogg, claims he's dying of a dread disease and announces plans to raise $100,000 for a new children's hospital. Knowing he'll ultimately be exposed, he plans to disappear and leave Uncle Jesse holding the bag.
Daisy accidentally gets her notebook, containing notes for the church play she is directing, switched with a notebook containing the criminal activities of mobster "Nervous" Norman Willis, who comes to Hazzard to collect payment due from Boss. When Willis finds out his notebook is switched, he begins tracking down Daisy, fearing she will take the notebook to the authorities.
Boss loses patience with Enos' continual reluctance to pursue the Dukes and, after demoting him to a civilian job, hires ill-tempered Billy Joe Coogan to replace him. After easily capturing Bo and Luke, Coogan demands Boss pay a $150,000 fee for services rendered, or else he and his cronies will blow up Hazzard Dam.
Jonas Jones, Cooter's friend and an ex-convict, is falsely blamed for hijacking Boss's expected shipment of automobile parts (which, of course, he plans to sell at inflated prices). Knowing that Jonas could spend years in prison for a crime he didn't commit, Cooter decides to take the fall. When they find out that the auto parts distributor is the front for a hijacking operation, the Dukes go undercover to infiltrate the scheme.
Boss hires a pair of fortune-telling con artists to steal the daily receipts at the Boar's Nest, then pin the blame on waitress Daisy. The fortune tellers are also involved in Boss' cover-up attempt to conceal his having cashing in on the Hoggs' bonds when Lulu asks to do so.
Waylon Jennings finally shows more than just his hands on TV in this cameo appearance. During a visit to Hazzard County, Ol' Waylon helps Bo and Luke foil Boss's plan to frame the Dukes for hijacking Waylon's traveling museum.
Luke drinks water laced with a personality-altering drug and becomes an abusive thug. While Luke conspires with a pair of criminals to rob Hazzard Bank, Bo, Daisy and Uncle Jesse race against time to demand answers from the research firm that developed the drug.
A computer salesman sells Boss a robot, which easily catches the Dukes. In the aftermath, Rosco loses his job for repeated mistakes. Later, the Dukes learn that the salesman is an embittered ex-computer programmer who had stolen the robot and plans to steal it back for ransom.
Daisy wins a cache of prizes after becoming the 1 millionth customer at the Capitol City Department Store. But the contest is a phony and the prizes were stolen, all as a part of Boss' latest scheme to frame the Dukes. But when he bumps his head and develops amnesia, his associates kidnap him, fearing he will reveal their scam to the authorities.
During a movie shoot in Hazzard County, movie star Brock Curtis is involved in an accident, and Bo and Luke save his life. The grateful Brock hires the Dukes as stunt doubles for his latest movie, to be shot in Hollywood. There, Bo and Luke learn the accident was not, and that someone is out to kill Brock to collect an insurance payment. Meanwhile, Boss and Rosco, who have accompanied the Dukes to Hollywood, try to break into movies, but are foiled at every turn. However, the producers see promise in Flash.
Bo and Luke are arrested in Osage County on false traffic charges and placed on the county's road gang. Outraged Hazzard residents pressure Boss and Rosco to negotiate with old enemy Cassius Claiborne for the Duke boys' freedom. Boss agrees to exchange moonshine contracts for the release of Bo and Luke, only to find himself and Rosco arrested and put on the road gang as well. It's all a part of Claiborne's plan to exploit Boss' voracious appetite to swindle him out of everything he owns.
Boss learns that Uncle Jesse's grandfather, Jeremiah Duke, had signed over the farm to his great grandfather, Thaddeus Hogg, and demands the deed to the farm. While the Dukes pack their things, Jesse discovers an old diary and reveals the truth - told in a flashback to the 1880s.
A pair of escaped convicts, including an ex-Marine that resented serving under Luke's command, force the Dukes to assist them in escaping the authorities. Their efforts involve holding Bo and Luke at gunpoint and making them drive through a forest fire.