When Jacob is fleecing Choo-Choo at cards, he plays with an Ace of Clubs, an Ace of Diamonds, and a Queen. When Choo-Choo picks a card, it is the Ace of Spades, and the Spade on the card is much larger than the other symbols. Since Jacob can manipulate the cards so that Choo-Choo always loses, this might be intentional. When Choo-Choo realizes he lost, we then see all three cards face-down. When they are turned over, only aces are shown, no queen.
As the outlaws are riding out of the town, Right after they shoot the sheriffs wife and drop his son the camera switches to an roof top shot. You can see the shadow of the camera on the ground. lower right of the screen.