When Mark Hand suggests having sex to Barbarella, she expects him to have "pills" for it, even though he is not from Earth. Later she is shocked that Dildano has them because only Earth people know of them.
When the Earth President greets Barbarella, he raises his left hand palm outward. Barbarella responds with her right hand palm outward. However, when the President farewells her, he does so with his right hand palm outward, and Barbarella responds with her right hand palm outward as well.
When Barbarella encounters a redheaded woman smoking "Essence of Man" from an enormous hookah and settles down on a pillow next to her, the redhead draws on the hookah again. She is holding the tube with both hands, her left hand on top. The next shot is a close-up on her, but she is suddenly holding the tube with her right hand only.
After Barbarella faints from being attacked by the "tooth dolls," several wounds on her legs and arms are visible. When she gets into the iceboat immediately after being rescued, the wounds have disappeared.
It is established that Barbarella needs a Tongue Box, a device attached to the bracelet she wears on her left wrist, to understand the spoken language of Sogo. Barbarella loses the bracelet after the Excessive Machine scene, but she still understands the Great Tyrant, Pygar and the Sogoites speaking through the Tyrant's monitor.
During the opening credits, Barbarella flies around in her spaceship . In a close-up, as she removes her gloves, her reflection appears in the Plexiglas she was lying on to give the illusion of floating.
When Barabarella and Pygar are attacked by the leather guards in the labyrinth, Barbarella tells Pygar to shoot her gun. "To the right!" she calls out. Pygar swivels left and shoots, hitting the guard dead on. Since Pygar is blind, he had only her instructions to go on.