When Mark Hand suggests having sex to Barbarella, she expects him to have "pills" for it, even though he is not from Earth. Later she is shocked that Dildano has them because only Earth people know of them.
When the Earth President greets Barbarella, he raises his left hand palm outward. Barbarella responds with her right hand palm outward. However, when the President farewells her, he does so with his right hand palm outward, and Barbarella responds with her right hand palm outward as well.
After Barbarella faints from being attacked by the "tooth dolls," several wounds on her legs and arms are visible. When she gets into the iceboat immediately after being rescued, the wounds have disappeared.
When Barbarella is flying around in her spaceship in the opening credits, in a close-up as she removes her gloves you can see her reflection in the Plexiglas she was lying on to give the illusion of floating.
When Barabarella and Pygar are being attacked by the leather guards in the labyrinth, Barbarella tells Pygar to shoot her gun. "To the right!" she calls out. Pygar, however, swivels left and shoots, hitting the guard dead on. Since Pygar is blind, he had only her instructions to go on.
At the beginning of the movie when Barbarella is about to remove her tunic, you can see that her spacesuit helmet is not attached at the neck allowing you to see her neck so the spacesuit is not air tight.