The film is set in 1966 or 1967, but some characters have back stories which would put them in older eras. Sir James Bond Senior appears to be in his mid-50s, but is said to have received the Victoria Cross at the Siege of Mafeking (1899-1900), which would put his age closer to 90. Mata Bond is portrayed as a 20-something, but she should be at least 50 since she is supposed to be the daughter of Mata Hari who was executed in 1917.
When Evelyn Tremble looks at Q's video wristwatch, it shows a clip of Vesper Lynd. This was a post-production error. It was supposed to show Bayldon, who stands in the background looking at the face of a complimentary wristwatch. This mistake caused a joke to be lost: Tremble says, "This is amazing, it's like you're in the same room" because Bayldon is indeed in the same room.
When David Niven (Sir James Bond) introduces himself to Moneypenny, he says "... I'm partial to Jasmine Tea." MoneyPenny: "Jasmine tea, Sir?" Bond: "Lapsang Soochong". These are two very different teas. Since Bond (and Niven) would certainly know the difference, this may be an intentional joke, e.g. a reference to the "real" Bond's "Martini... shaken, not stirred", or (as much of the dialog was improvised) a cast in-joke.
When Vesper Lynd is on the rotating bed with Evelyn Tremble, persuading him to put on costumes for her to photograph him in, when she gets up to use the still camera on the tripod her robe abruptly changes color from a pale, almost white, pink to a darker pink and the decorations in her hair change from feathers to hearts.
When Terence Cooper is being conditioned to resist women, Lorelei the skin diving girl is shown to be in front of him but in a long shot of Cooper, she can clearly be seen in the background behind him, yet in the next shot, she approaches him from the front.
When Andress seduces Sellars in the casino she grip the handle of an old fruit machine.Initially it has a red round handle but moments later the rounded knob is black. A second look shows it to be a completely different machine.
When Sir James Bond Senior and Miss Moneypenny Junior are running down the hall in SMERSH headquarters, they are holding hands with Sir James on Moneypenny's right hand side, then not holding hands (with Sir James now on the left): they change sides three times before they reach the the blue door.
When the French Legionairre comes in in the final fight scene he hits a man and immediately writhes his hand in pain and say "Merde!" Sir James Bond asks him what he means and he translates it a "Ooooch!". In actual fact 'Merde' is the French translation of 'sh*t'. He does exactly the same when we later see him.
M tells his companions that Sir James had to lure Mata Hari, his true love, across the Spanish frontier into France. Of course, this never happened, and the real Mata Hari never entered France by crossing the Spanish frontier. Mata Hari was last in Spain in November 1916. She was arrested in Paris in February 1917 after the Germans exposed her deliberately to the French as a double-agent because the intel she was providing them was deemed useless (she was recruited by France to seduce Crown Prince Hohenzollern; the French believed mistakenly that he was a commander in his father's army). However, neither the French or the British could produce definite evidence of her spying for Germany at her trial.
When Bond (Niven) and Moneypenny are trying to escape the secret SMERSH base, we suddenly cut back to them and see that Mata Bond and Cooper have wordlessly joined them. Although Mata Bond was put in the same base earlier, Cooper was last seen much earlier in the film safe at MI5, and his presence in the base id never explained.
In the Baccarat scene between Peter Sellers and Orson Welles, in the long shots both characters are surrounded by a crowd of extras, mostly women. In the close-ups of Sellers alone, the background shows only a few people, mostly men; a woman in a red dress appears in two shots but not in others, while Ursula Andress is in the shots that do not have the red dress. Several of the characters appear motionless and slightly blurred, possibly a still back-projection or montage.
After Vesper is kidnapped outside the casino, James Bond alias Evelyn Tremble enters his Lotus Formula 3 to find her, but in the next film shot, Bond alias Tremble is in Le Chiffre's psychological torture room. How was he caught? (There is probably a missing scene from the script, never filmed due to production difficulties: see trivia.)