John Saxon later claimed that Gene Corman had more to do with Queen of Blood than Roger. Saxon estimated that his scenes were shot in seven to eight days and that Dennis Hopper "was trying very hard to keep a straight face throughout" during the making of the film.
Basil Rathbone was paid $1,500 to act for a day and a half on Queen of Blood, and $1,500 for half a day on Voyage to the Prehistoric Planet (1965), another film that incorporated Russian film footage. Rathbone ended up working overtime and missed a meal. The Screen Actors Guild demanded overtime pay, plus a fine for the meal violation, but producer George Edwards produced footage that showed the delay was because Rathbone had not memorized all his lines and insisted on skipping lunch.
Czech actress Florence Marly was a personal friend of director Harrington. He later said that he had to fight with Roger Corman in order to hire her "because she was an older woman. Harrington would say, "I'm sure he had some bimbo in mind, you know? So I fought for Marley because I felt she had the required exotic quality that would work in the role."Harrington also said Dennis Hopper "was like a part of my little team by then," so he agreed to also appear.
Harrington had made his name with the feature Night Tide, which impressed Roger Corman enough to offer the director a film project. "Of course, I would like to do a more individual film than Queen of Blood", said Harrington at the time, "but I can't get the financing. However, the film is entertaining, and I feel I was able to say something within the context of the genre."