Originally planned as the pilot film for the Batman (1966) TV series, the movie was instead produced between the show's first and second seasons. The producers took advantage of the larger budget to have a number of new Bat-gadgets constructed, such as the BatBoat.
The BatBoat was built especially for the film by the Glastron boat company. In exchange for their cooperation, the producers agreed to hold the film's world premiere in Austin, Texas, Glastron's headquarters.
In the final fight scene, a stuntman playing one of the villains' henchmen dove into the water and hit his head on a metal stud at the bottom of the pond. He was knocked unconscious and had to be rushed to the hospital.
Julie Newmar (Catwoman in the TV series) does not appear in this film because she did not know about it and had signed to do another project. By the time she was informed, she could not get out of the other commitment in time to do this movie.
Scenes shot in the arch-criminals' headquarters lair were filmed at an angle. Rumors at the time were that this was intentional and was meant to show that the four (Catwoman, Penguin, Joker, and Riddler) were crooked.
The original trailer includes specially-shot footage of the 4 supervillains outlining their plans for the Dynamic Duo. Still frames from these sequences are visible when Batman and Commissioner Gordon watch a closed-circuit TV update on villains at large. The trailer also includes specially-shot footage of Batman and Robin addressing the audience about their first motion picture.
At the end of the film one of the delegates is seen banging his shoe on the table while yelling. This is a parody of Nikita Khrushchev's famous behavior during a debate in the United Nations General Assembly in 1960.
The faking of sea outside a phony yacht window was a clever ruse inspiring a Hogan's Heroes (1965) scene in which a kidnapped general is tricked into thinking he is aboard a plane flying at night to England.
A follow up film was at one point considered. The film would have been released between seasons two and three, and would have been used to introduce Barbara Gordon/Bat Girl, and make use of a Batplane. Due to waning interest in the series during season two, which resulted in budget cuts, plans for a second film were scratched.
The Penguin's line "We shall hang together, or most assuredly we shall all hang separately" was a humorous phrase spoken by Benjamin Franklin when he was in danger of being accused of high treason by his fellow delegates.
The opening criminal lair, "Ye Olde Benbow Tavern" is an allusion to name of the tavern in which the the Robert Louis Stevenson novel "Treasure Island" begins, "Admiral Benbow Inn". Commodore Schmidlapp is abducted by the fiendish foursome. The next novel by R. L. Stevenson is "Kidnapped".
Dick Grayson appears outside of his Robin persona only twice and very briefly in the film. First, at the very beginning, and later when Bruce returns to Wayne Manor after being kidnapped. Dick's only spoken lines are in the latter scene.
The song played by the Salvation Army Band as Batman tries to dispose of the bomb is Bringing In The Sheaves, a popular Protestant Hymn. Various versions of the song can be heard in several other films and TV episodes.
The movie was originally intended to be an introduction to the TV series. When the series wound up being produced and aired months ahead of schedule, the movie was made to cash in on the show's popularity.