Daniel Boone is commissioned by George Washington to build a fort in the territory of Kentucky. The British are talking to the Indian Nations and trying to win them over to their side and it's Washington's hope that Boone and his people will be able to stop their advancement. While scouting the land out Boone helps a Cherokee out who is being hunted by Shawnee...his name is Mingo, an Oxford educated Half-breed, and he and Boone become fast friends. Boone is captured by Dark Panther of the Shawnee and he learns of their plans to attack Boone's fort in Pennsylvania ...
A dance at Fort Boonesborough is interrupted by Flathead Joseph, a halfbreed slave trader. He has a young Indian girl with him whom he claims is a Cherokee Princess...and a white girl. Her name is Tekawitha McLeod and she is the daughter of the Cherokee Chief Menewa. When Daniel confronts Menewa about this he admits she is white and taken from a raiding party but he wants her back. Daniel refuses when he realizes he knew her family. The Indians are determined to fight to get her back and the settlers of Boonesborough are just as determined to fight to keep her.
Mingo(Ed Ames), wanted DEAD! Daniel Boone(Fess Parker) must capture his friend Mingo and bring him to justice before all Kentucky erupts in war. Daniel's friend Yadkin(Albert Salmi) is a trusted friend to all in Booneborough and among the Cherokee. Yadkin had canoed up river and while meeting with a Cherokee trading party when they were ambushed by a Creek war party. Yadkin was found floating down river by Daniel's son Israel(Darby Hinton). With Cincinnatus(Dal McKennon) removing the slug(bullet) and Rebecca Boone(Patricia Blair) nursing him, he began to pull through....
As Daniel and Yadkin try to find the latest poachers, they meet a Welsh widow with three children who is determined to settle in Shawnee territory in spite of the fact that the tribe has been hostile to settlers in the recent past.
Daniel and Jemima have planned a pleasant father daughter outing in the wilderness, but things take a nightmarish turn when Daniel accidentally cuts himself with an axe and a band of Native Americans kidnaps his daughter.
Lac Duquesne and Daniel Boone were friends once but now Duquesne has turned to river piracy to make his living. When Duquesne steals 5 cases of rifles from a small riverboat on their way to Boonesborough it becomes Daniel's responsibility to get them back before Duquesne can sell them to the Shawnee. Daniel, Mingo and Yadkin set out in pursuit of Duquesne and the rifles.
Major Wellington of the British forces asks Boone to assist him in the rescue of 4 British officers who were trying to negotiate a treaty with the local Indians. Boone agrees to go, but Wellington kills Boone and assumes his identity. Wellington's scheme to negotiate a treaty comes undone when Boone arrives unexpectedly at the village Wellington is in. The Indians don't know who to believe, and set the two men against each in a series of trials to discover the truth.
Simon Girty and his three boys are pelt thieves out to rob Boonesborough of their furs. Daniel, Yadkin and Israel are transporting the furs to Salem to sell them. It's not long before they fall prey to the thieving family and lose their furs, rifles and provisions. Outgunned, Daniel will have to come up with a clever plan to outsmart the Girtys to get their furs back.
Russell who has conspired with MacAnders to hide the payroll of massacred British unit arrives from England, kills MacAnders and enlists Daniel to take him and two others to the massacre site while Daniel is visited by MacAnders' ghost.
A woman accused of witchcraft and her small son flee from a mob and escape to Boonesborough. They are found in the wilderness and nursed back to health by Daniel and Rebecca, but when her husband arrives the hysteria begins again. It is up to Daniel to use courage and wisdom to stand down the charges of witchcraft and help this family of innocent strangers.
A British officer is kidnapped by a group of Shawnee Indians intent on revenge because of his involvement in a massacre. He escapes and Boon finds him and get involved in reconciling enormous hatred on both sides.
Daniel and Mingo come across the massacre of a Cherokee camp. Mingo is outraged but Boone calms him enough to focus on seeking these men out and bringing them to justice before Cherokee warriors extract their revenge.
While out hunting, Daniel and Cincinnatus come upon Timothy the peddler just after he has scared off a group of Shawnee. In Boonesborough they learn he's looking for his daughter Becky, Daniel's wife, whom he abandoned when he walked out on her mother many years earlier in Ireland. Their reunion is not a happy one.
A French officer named Michelet conspires with the various Indian tribes to lay siege to Boonesborough. The Indians appear to have overwhelming numbers and the cutoff townspeople are desperately short on supplies.
Boone and the people of Boonesborough hole up in the fort using every means possible to fend off the attacking Indians while hoping that a promised British relief force will reach them before it's too late.
A man is found dead by a group of travelers, one of them hears a strange noise and believes it to be the devil. Daniel and Mingo believe a far more mundane explanation is at hand, and investigate to prove their point.
Israel accidentally startles a passing peddler's horse and his goods are ruined. Daniel agrees to compensate him for his loss but matters are complicated when he steals a grieving Indian women's mourning doll.
Jericho and Sumah, the daughter of the Indian chief, want to marry. Daniel proposes a trial engagement. For a month Jericho will live with the Indians and Sumah will live with Boone's family and see if each can adapt to the other culture.