Bugs' exercise routine is a nod to the emerging fitness fad promoted by Jack La Lanne.
Bugs had previously joined the USMC in the 1943 short "Super Rabbit."
Porky Pig, Sylvester the Cat and Tweetie Bird are all referenced in this short.
The Acme Company also makes eye charts, according to Bugs' eye exam.
Bugs being able to read the eye chart in its entirety, including the microscopic writing at the bottom regarding the manufacturing of it, is a joke about how good of eyesight rabbits have based on their steady diet of primarily carrots and how it has been said the consumption of carrots help promote great eyesight.