- much lengthier (and always on-camera) interstitials from Deems Taylor, especially for the then-revolutionary "Rite of Spring" sequence.
- Footage of the musicians exiting and re-entering the bandstands immediately before and after the intermission. The sequence after the intermission features an impromptu jam session by the on-screen musicians.
- No on-screen credits, save one title card which displayed the film's name, the copyright notice, "In Technicolor", the MPPDA approval certificate, and the RCA Sound System logo. The production credits were featured in a specially prepared collectible program booklet available for purchase by roadshow performance attendees. Since the first editing of the film after its initial roadshow release, none of this extra material (except part of the intermission used for the 50th Anniversary's end credits sequence) has been seen publicly until the 60th Anniversary release.
- it features a monophonic soundtrack in place of the four-track "Fantasound" soundtrack
- The RKO distribution tag is added to the front of the film.
- The film's single title card (played over the intermission) now also features the RKO logo.
- The edits made to the "Pastoral Symphony" that are present in all post-1969 prints of the film. See above.
- The addition of an end credits sequence played against footage of the on-screen orchestra exiting the stage, as first seen preceding the intermission in the Roadshow Version. The 1990 version has been released on VHS and LaserDisc. No other version of Fantasia features a credits sequence (the credits were made available to the 1940-1941 roadshow patrons in a specially prepared commemorative booklet).
- an alternate broom-chopping from "Sorcerer's Apprentice" that originally occurred on-screen, rather than off-screen.
- an alternate angle version of the crocodile/hippo encounter from "Dance of the Hours."