 | Rex Lease | ... | Kit Cardigan / John C. Cardigan [Ch. 1] |
| Lona Andre | ... | Belle Meade |
| William Farnum | ... | James Fitzpatrick |
| Ruth Mix | ... | Elizabeth Custer [Chs. 1,3, 8-11,15] |
| Jack Mulhall | ... | Lieutenant Cook |
| Nancy Caswell | ... | Barbara Trent |
| George Chesebro | ... | Lieutenant Frank Roberts (as George Chesboro) |
| Dorothy Gulliver | ... | Red Fawn |
| Frank McGlynn Jr. | ... | General George A. Custer |
| Helen Gibson | ... | Calamity Jane [Chs. 4-7, 10] |
| Josef Swickard | ... | Major Henry Trent MD |
| Chief Thundercloud | ... | Young Wolf |
| Reed Howes | ... | Tom 'Keen' Blade |
| Bobby Nelson | ... | Bobby Nelson (as Bobbie Nelson) |
| Robert Walker | ... | Henchman Pete |
| Marty Joyce | ... | Buzz |
| Milburn Morante | ... | Buckskin (as Milburn Moranti) |
| George Morrell | ... | Sergeant Major Flannigan |
| Creighton Hale | ... | Hank |
| Ted Adams | ... | Barney (henchman) / Buffalo Bill Cody [Chs. 6-7] |
| Carl Mathews | ... | Chief True Eagle [Chs.1, 15] / Curly, Custer's orderly |
| Allen Greer | ... | Wild Bill Hickok [Chs.7-10] |
| High Eagle | ... | Chief Crazy Horse |
| Howling Wolf | ... | Chief Sitting Bull |
| Chief John Big Tree | ... | Medicine Man [Ch. 9] (as Big Tree) |
| Iron Eyes Cody | ... | Chief Brown Fox (as Iron Eyes) |
| Chief Thunderbird | ... | Rain-in-Face - Young Wolf's henchman (as Chick Davis) |
| William Desmond | ... | Wagon master [Ch. 1] (as Bill Desmond) |
| Walter James | ... | Judge Hooker [Chs.12-14] |
| James Sheridan | ... | Henchman Jim |
| Ken Cooper | ... | Henchman - Ch. 5 |
| Budd Buster | ... | Maj. Ware [Chs. 8-9] |
| Carter Wayne | ... | Orderly 'Striker' Martin |
| Cactus Mack | ... | Lieutenant Weir [Chs. 10, 15] |
| Barney Furey | ... | Sergeant Major Peters (as Barney Fury) |
| Lafe McKee | ... | Captain Benteen [Ch. 14] |
| Franklyn Farnum | ... | Major Marcus Reno [Chs. 11, 14, 15] |
| Ed Withrow | ... | Blue Crow (murdered Indian) [Chs. 10-11] |
| Artie Ortego | ... | Indian [Ch. 5] / Quirk (halfbreed henchman) [Chs. 7, 13] |
| rest of cast listed alphabetically: |
| William Bartlett | ... | Renegade (uncredited) |
| Humming Bird | ... | Renegade (uncredited) |
| Fred Burns | ... | Wagon Driver (uncredited) |
| Yakima Canutt | ... | Renegade / Indian (uncredited) |
| J.W. Cody | ... | Indian (uncredited) |
| Little Eagle | ... | Renegade (uncredited) |
| Buddy Fisher | ... | Renegade (uncredited) |
| Walter Gable | ... | Renegade (uncredited) |
| Gaston Glass | ... | Joe - Bartender (uncredited) |
| Herman Hack | ... | Henchman (uncredited) |
| William Hunt | ... | Renegade (uncredited) |
| Charles Hunter | ... | Renegade (uncredited) |
| Herb Jackson | ... | Renegade (uncredited) |
| Wilbur McGaugh | ... | Man Who Brings Gun [Ch. 12] (uncredited) |
| Lone Pine | ... | Indian (uncredited) |
| Potter Poe | ... | Crow Scout (uncredited) |
| Whitten Sovern | ... | Renegade (uncredited) |
| Red Star | ... | Indian (uncredited) |
| Mabel Strickland | ... | Mabel - Pioneer Woman (uncredited) |
| Swift Eagle | ... | Indian (uncredited) |
| Bill Thompson | ... | Renegade (uncredited) |
| Tall Tree | ... | Renegade (uncredited) |
| Chief White Feather | ... | Renegade (uncredited) |
company.